1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily Neurotrophic Factors
  4. Growth Differentiation Factor GDNF family
  5. Growth Differentiation Factor 15 (GDF-15)
  6. GDF-15 蛋白, Mouse (HEK293, His-Flag)

GDF-15 蛋白, Mouse (HEK293, His-Flag)

目录号: HY-P700293
COA 产品使用指南 技术支持

GDF-15 蛋白通过与其受体 GFRAL 结合来调节食物摄入、能量平衡和体重。这会激活脑干中表达 GFRAL 的神经元,在压力期间触发中央臂旁核和杏仁核的“紧急回路”。GDF-15 蛋白, Mouse (HEK293, His-Flag) 是重组的 GDF-15 蛋白,由 HEK293 表达,带有 N-8*His, N-Flag 标签。

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SPR 检测服务   重组蛋白定制服务

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1390 In-stock
50 μg ¥3900 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The GDF-15 protein regulates food intake, energy balance, and body weight by binding to its receptor GFRAL.This activates GFRAL-expressing neurons in the brainstem, triggering "emergency circuits" in the central parabrachial nucleus and amygdala during stress.GDF-15 Protein, Mouse (HEK293, His-Flag) is the recombinant mouse-derived GDF-15 protein, expressed by HEK293 , with N-8*His, N-Flag labeled tag.

研究背景

GDF-15 蛋白在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。它通过与其受体 GFRAL 结合并激活位于脑干后区和孤束核的表达 GFRAL 的神经元来实现这一点。这种激活随后触发臂旁核和中央杏仁核内神经元的激活,这些神经元是“紧急回路”的一部分,负责在压力条件下形成进食反应。此外,GDF-15 蛋白可抑制肝细胞上的生长激素信号传导,并形成二硫键连接的同型二聚体。它还与 GFRAL 相互作用,作为配体通过与 RET 的相互作用促进 GDF15 内化和细胞信号传导。

生物活性

Measured by its ability to induce SRE reporter activity in HEK293 human embryonic kidney cells overexpressing GFRAL and RET. The typical ED50 is <4.1 ng/mL.

种属

Mouse

表达系统

HEK293

标签

N-8*His;N-Flag

蛋白编号

Q9Z0J7 (S189-A303)

基因 ID
蛋白结构
N-term
8*His-Flag
GDF-15 (S189-A303)
Accession # Q9Z0J7
C-term
蛋白长度

Full Length of Mature Protein

中文名
GDF-15 蛋白
同用名
Growth Differentiation Factor 15; Macrophage inhibitory cytokine 1; GDF-15; MIC-1; NAG-1; PLAB; PTGFB; Gdf15; Sbf
氨基酸序列

SAHAHPRDSCPLGPGRCCHLETVQATLEDLGWSDWVLSPRQLQLSMCVGECPHLYRSANTHAQIKARLHGLQPDKVPAPCCVPSSYTPVVLMHRTDSGVSLQTYDDLVARGCHCA

分子量

14-16 KDa

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

内毒素含量

Less than 1 EU/µg as determined by LAL test.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GDF-15 Protein, Mouse (HEK293, His-Flag)
目录号:
HY-P700293
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