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  3. SNCA 蛋白, Human

SNCA 蛋白 是一种异常聚集形成路易小体的蛋白质。SNCA 蛋白 通过基因拷贝数增加 (如三倍体) 或突变导致神经元分化和成熟障碍、抑制神经突生长、降低神经元电活动,并通过干扰自噬-溶酶体途径 (ALP) 促进细胞外毒性颗粒 (如低聚体) 的释放,进而诱导炎症反应和邻近细胞损伤。SNCA 蛋白, Human 是重组的 SNCA 蛋白,由 E. coli 表达,不带标签。

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5 μg ¥300 In-stock
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  • 生物活性

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描述

SNCA Protein is a protein abnormally aggregated to form Lewy bodies. SNCA Protein causes neuronal differentiation and maturation disorders, inhibits neurite outgrowth, reduces neuronal electrical activity through increased gene copy number (such as triploidy) or mutation, and promotes the release of extracellular toxic particles (such as oligomers) by interfering with the autophagy-lysosomal pathway (ALP), thereby inducing inflammatory responses and damage to neighboring cells. SNCA Protein, Human is a recombinant SNCA protein expressed by E. coli without a tag.

研究背景

SNCA Protein 属于突触核蛋白 (Synuclein) 家族。SNCA Protein 是一种蛋白质,其异常聚集形成路易小体[1]。SNCA Protein 通过基因拷贝数增加 (如三倍体) 或突变导致神经元分化和成熟障碍、抑制神经突生长、降低神经元电活动,并通过干扰自噬-溶酶体途径 (ALP) 促进细胞外毒性颗粒 (如低聚体) 的释放,进而诱导炎症反应和邻近细胞损伤[1][2][3]。SNCA Protein 主要在中枢神经系统的神经元中表达,尤其在多巴胺能神经元中富集[1]。SNCA Protein 的正常活性与突触囊泡的动态调节和神经递质释放相关,但其病理状态下的异常聚集会破坏线粒体功能、诱导氧化应激,并通过外泌体等途径在细胞间传播毒性,加剧神经退行性病变[1][2][3]。SNCA Protein, Human 由人类 SNCA 基因编码,其蛋白包含 140 个氨基酸残基,可通过翻译后修饰(如磷酸化、泛素化) 影响其聚集倾向[1][4]

生物活性

Measured by its ability to inhibit cell proliferation of A549 cells. The ED50 for this effect is typically 15.86 ng/mL, corresponding to a specific activity is 6.31×104 units/mg.

  • Measured by its ability to inhibit cell proliferation of A549 cells. The ED50 for this effect is typically 15.86 ng/mL, corresponding to a specific activity is 6.31×104 units/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P37840-1 (M1-A140)

基因 ID
蛋白结构
N-term
SNCA (M1-A140)
Accession # P37840
C-term
蛋白长度

Full Length of Isoform-1

中文名
SNCA 蛋白
同用名
Alpha-Synuclein; Non-A Beta Component of AD Amyloid; Non-A4 Component of Amyloid Precursor; NACP; SNCA; NACP; PARK1
氨基酸序列

MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA

分子量

Approximately 18.0 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

SNCA Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SNCA Protein, Human
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