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Blue Tetrazolium  (Synonyms: Tetrazolium blue)

目录号: HY-D0007
产品使用指南

Blue Tetrazolium (Tetrazolium blue),作为微生物研究用的蓝色染料,会在使用时还原成蓝色四唑甲臜 (BTF)。Blue Tetrazolium 可用于测定酵母细胞中琥珀酸脱氢酶 (SDH) 活性,作为底物被还原。用 DMSO 从细胞中提取 BTF,测试 BTF 的吸收光谱。BTF 具有广谱波长 (480-600 nm),在 540 nm 处可见最大吸光度。

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Blue Tetrazolium Chemical Structure

Blue Tetrazolium Chemical Structure

CAS No. : 1871-22-3

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH)[1].

分子量

727.64

Formula

C40H32Cl2N8O2

CAS 号
中文名称

氯化四唑蓝

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

Blue Tetrazolium 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Blue Tetrazolium
目录号:
HY-D0007
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