Endo-β-N-acetylglucosaminidase F2  (Synonyms: Endo F2)

Endo-β-N-acetylglucosaminidase F2 (Endo F2), a highly specific endoglycosidase, cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. Endo F2 is not active on hybrid or tri- and tetra-antennary oligosaccharides^[1].

others

使用方法
1）10 μg 的糖蛋白，加入 1 μL 的 500 mM Sodium acetate (pH=5.5)，用去离子水定容到 10 μL；
2）加入 1-2 μL 的 Endo F2，轻轻吹打混匀；
3）37°C 孵育1h；
4）用 SDS-PAGE 或质谱分析；
注：孵育时间或酶量需要根据实际情况进行优化。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

液体

Colorless to light yellow

糖苷内切酶 F2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. R B Trimble, et al. Identification of distinct endoglycosidase (endo) activities in Flavobacterium meningosepticum: endo F1, endo F2, and endo F3. Endo F1 and endo H hydrolyze only high mannose and hybrid glycans. J Biol Chem. 1991 Jan 25;266(3):1646-51.  [Content Brief]