2. MAPK/ERK Pathway Stem Cell/Wnt
  3. ERK
  4. Endothelin-1 (1-31) (Human) TFA

Endothelin-1 (1-31) (Human) TFA 

目录号: HY-P4159A 纯度: 99.07%
COA 产品使用指南

摩尔计算器

Endothelin-1 (1-31) (Human) TFA 是一种有效的血管收缩剂和血压升高剂。Endothelin-1 (1-31) (Human) TFA 来源于 chymase 对大 ET-1 的选择性水解。

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Custom Peptide Synthesis

Endothelin-1 (1-31) (Human) TFA Chemical Structure

Endothelin-1 (1-31) (Human) TFA Chemical Structure

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥2300
In-stock
5 mg ￥7000
In-stock
10 mg   询价  
50 mg   询价  

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Customer Review

Other Forms of Endothelin-1 (1-31) (Human) TFA:

Endothelin-1 (1-31) (Human) TFA 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 ERK 亚型特异性产品:

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ERK ERK1 ERK2 ERK5 ERK8

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Endothelin-1 (1-31) (Human) TFA is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) TFA is derived from the selective hydrolysis of big ET-1 by chymase[1].

体外研究
(In Vitro)

Endothelin-1 (1-31) (Human) (100 pM-100 nM; 24 h) TFA 诱导人系膜细胞增殖[2]。
Endothelin-1 (1-31) (Human) (100 nM; 0-10 min) TFA 在人系膜细胞中诱导 ERK 激活[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Endothelin-1 (1-31) (Human) TFA 相关抗体:

Cell Proliferation Assay[2]

Cell Line: Human mesangial cells
Concentration: 100 pM-100 nM
Incubation Time: 24 h
Result: Caused an increase in [3H]-thymidine incorporation into the cells in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: Human mesangial cells
Concentration: 100 nM
Incubation Time: 0, 5, 10, 15 and 30 min
Result: ERK activities rapidly increased 2.45-fold at 5 min and peaked at 10 min. The activities of both ERKs rapidly declined, returning to the baseline control value 30 min after stimulation.
体内研究
(In Vivo)

ET-1 (1-31) (100 nM; single dose) TFA 诱导小鼠肠系膜动脉收缩。收缩可能是由 ETA 受体介导的，并可能随着年龄的增长而增加。在目前的慢性糖尿病的情况下，男性和女性之间存在明显差异[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice, Streptozocin (HY-13753)-induced diabetic model[1]
Dosage: 100 nM
Administration: In the organ bath, single dose
Result: In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ETA receptor inhibitor.
分子量

3742.18

Formula

C164H237F3N38O49S5
性状

固体

颜色

White to off-white

Sequence

Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)
Sequence Shortening

CSCSSLMDKECVYFCHLDIIWVNTPEHVVPY (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
细胞实验： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献

Endothelin-1 (1-31) (Human) TFA 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Endothelin-1 (1-31) (Human)ERKExtracellular signal regulated kinasesvasoconstrictorhypertensivemesangial cellInhibitorinhibitorinhibit

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产品名称：
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目录号：
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