2. MCE Kits
  3. Cell Biology
  4. Cell Culture
  5. Dissociation Reagents
  6. 0.25% Trypsin-EDTA (1x), phenol red

0.25% Trypsin-EDTA (1x), phenol red 

胰蛋白酶-EDTA (0.25%)，含酚红

Cat. No.: HY-K3007
Manual SDS

MCE 0.25% Trypsin-EDTA (1x), phenol red 由胰蛋白酶干粉与 EDTA 混合溶解而成，可广泛应用于细胞解离、常规细胞培养传代及原代组织解离，使组织或贴壁细胞分散成单个细胞。

0.25% Trypsin-EDTA (1x), phenol red

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
100 mL ￥110 In-stock
100 mL * 5 ￥330 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE 0.25% Trypsin-EDTA (1x), phenol red consists of dried trypsin powder (a mixture of proteases from porcine pancreas, irradiated and sterilized) dissolved in EDTA. MCE Trypsin hydrolyzes intercellular proteins and disperses primary tissues or adherent cells into individual cells, and can be widely used for cell dissociation, cell culture passaging and primary tissue dissociation.
Storage

-20°C, 2 years.
Protocol
Cell dissociation:

1. Aspirate the culture medium and wash the cells twice with sterile PBS, Hanks or serum-free culture medium to remove residual serum.

2. Add the pre-warmed trypsin-EDTA dissociation solution to cover the cells and incubate at room temperature for approximately 2 minutes. Note that the actual incubation time varies with the cell line used.

3. Observe under the microscope, and when ≥90% of the cells are detached, terminate the dissociation by adding twice the volume of serum-containing complete medium.

4. Centrifuge at 200×g for 5-10 minutes and resuspend the cell precipitates in complete medium containing serum for subsequent experiments.

Tissue dissociation:

Determine the optimal conditions based on relevant experience and literature, as the digestion time required varies from different tissues.
Components
Components HY-K3007 HY-K3007-500 mL
0.25% Trypsin-EDTA (1x), phenol red 100 mL 100 mL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
胰蛋白酶-EDTA (0.25%)，含酚红
目录号：
HY-K3007
需求量：
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