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  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Blocking Buffer
  6. Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×)

Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 

快速封闭液速溶颗粒 (100 mL of 1×)

Cat. No.: HY-K1027
Manual SDS

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 主要成分为鱼明胶蛋白，常用于 Western Blot 和 ELISA 的抗体封闭步骤，可在 15 分钟内完成封闭。

Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×)

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (2 pouches)   Apply now  
10 pouches ￥240 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein. Each pouch can be diluted to 100 mL 1× blocking buffer, in which the concentration of the protein is 5%. Containing Tween 20, this product is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

This product provides a convenient way to make blocking buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Prepare the working buffer

a.   Add 1 pouch of Rapid Blocking Buffer (TBS-T) Powder into the cleaned beaker, dissolve with 50 mL distilled water under a magnetic stirrer.

b.   Add distilled water to the solution in step 1 until the total volume is 100 mL. The final solution is 1× working buffer.

2.    Blocking step

a.   Place the membrane into the incubator box, add 10-20 mL 1× working buffer and incubate in the shaker incubator at room temperature for about 10 minutes.

b.   The blocked membrane can be used for the subsequent experiments.

Components
Components HY-K1027-10 pouches
Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 10 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
快速封闭液速溶颗粒 (100 mL of 1×)
目录号：
HY-K1027
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