1. Academic Validation
  2. Dipyrithione induces cell-cycle arrest and apoptosis in four cancer cell lines in vitro and inhibits tumor growth in a mouse model

Dipyrithione induces cell-cycle arrest and apoptosis in four cancer cell lines in vitro and inhibits tumor growth in a mouse model

  • BMC Pharmacol Toxicol. 2013 Oct 21;14:54. doi: 10.1186/2050-6511-14-54.
Yumei Fan Caizhi Liu Yongmao Huang Jie Zhang Linlin Cai Shengnan Wang Yongze Zhang Xianglin Duan 1 Zhimin Yin
Affiliations

Affiliation

  • 1 The Key Lab of Animal Physiology, College of Life Science, Hebei Normal University, Hebei Province, Shijiazhuang 050024, China. xlduan0311@163.com.
Abstract

Background: Dipyrithione (PTS2) is widely used as a bactericide and fungicide. Here, we investigated whether PTS2 has broad-spectrum antitumor activity by studying its cytotoxicity and proapoptotic effects in four Cancer cell lines.

Methods: We used MTT assays and trypan blue staining to test the viability of Cancer cell lines. Hoechst 33258 and DAPI staining were used to observe cell Apoptosis. Cell-cycle percentages were analyzed by flow cytometry. Apoptosis was assayed using Caspase-3 and poly (ADP-ribose) polymerase (PARP) combined with Western blotting. Student's t-test was used for statistical analysis.

Results: PTS2 inhibited proliferation in four Cancer cell lines in a dose-dependent manner. Treated cells showed shrinkage, irregular fragments, condensed and dispersed blue fluorescent particles compared with control cells. PTS2 induced cycle-arrest and death. Cleavage of caspase-9, Caspase-3, and PARP were detected in PTS2-treated cells. Antitumor activity of PTS2 was more effective against widely used Cancer drugs and its precursor.

Conclusions: PTS2 appears to have novel cytotoxicity and potent broad-spectrum antitumor activity, which suggests its potential as the basis of an Anticancer drug.

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