1. Academic Validation
  2. Alleviation of transient global ischemia/reperfusion-induced brain injury in rats with 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose isolated from Mangifera indica

Alleviation of transient global ischemia/reperfusion-induced brain injury in rats with 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose isolated from Mangifera indica

  • Eur J Pharmacol. 2013 Nov 15;720(1-3):286-93. doi: 10.1016/j.ejphar.2013.10.016.
G L Viswanatha 1 H Shylaja C G Mohan
Affiliations

Affiliation

  • 1 Department of Pharmacology, PES College of Pharmacy, Hanumanthanagar, Bangalore 560050, Karnataka, India. Electronic address: glv_000@yahoo.com.
Abstract

Present study was undertaken to evaluate the protective effect of 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (PGG) against transient global ischemia/reperfusion (I/R)-induced brain injury in rats. Sixty minutes of global ischemia, followed by 24h of reperfusion caused significant alterations in cognition and memory (p<0.01), significant deterioration of motor coordination, grip strength, and limb tone (P<0.01) associated with neurological deficit. In addition, significant decrease in catalase (P<0.01), and superoxide dismutase (SOD) (P<0.01) activities, increase in lipid peroxidation (P<0.01), depletion of reduced glutathione (GSH) (P<0.01), and increase in brain volume (P<0.01) was observed. Additionally, I/R insult has aggravated the cerebral infarct formation (P<0.01), and the histopathology of brain showed congestion of blood vessels, edema of brain parenchyma, leukocyte infiltration as signs of neuroinflammation, and necrosis of brain tissue. Interestingly, pre-treatment with quercetin (20mg/kg, i.p.), and PGG (5 and 10mg/kg, i.p.) for 7 days showed significant, and dose dependent protection against I/R-induced brain injury by alleviating all the behavioral, neurological, morphological, and histological changes induced by I/R. Besides, PGG is a well-known antioxidant, and its protective effect against I/R-induced brain injury is thought to be due to its potent antioxidant property.

Keywords

1,2,3,4, 6-Penta-O-galloyl-β-d-glucopyranose; Ischemia/reperfusion; Neuroprotection; Oxidative stress; Stroke.

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