2. Academic Validation
  3. Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC-MS/MS

Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC-MS/MS

  • Biomed Chromatogr. 2017 Mar;31(3). doi: 10.1002/bmc.3826.
Bingmu Fang 1,  Shihui Bao 2,  Shuanghu Wang 3,  Minle Chen 3,  Bingbao Chen 4,  Ke Su 4,  Congcong Wen 4,  Yunfang Zhou 3,  Xianqin Wang 5,  Yuepeng Jin 6
Affiliations

Affiliations

  • 1 Department of Hematology, The People's Hospital of Lishui, Lishui, 323000, China.
  • 2 The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
  • 3 Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
  • 4 Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.
  • 5 Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.
  • 6 The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
PMID: 27565758 DOI: 10.1002/bmc.3826
Abstract

In this work, a sensitive and selective UPLC-MS/MS method for determination of ardisiacrispin A in rat plasma was developed. Cyasterone used as an internal standard (IS) and protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 1083.5 → 407.1 for ardisiacrispin A and m/z 521.3 → 485.2 for IS. Calibration plots were linear throughout the range 5-2000 ng/mL for ardisiacrispin A in rat plasma. Mean recoveries of ardisiacrispin A in rat plasma ranged from 80.4 to 92.6%. The values of RSD of intra- and inter-day precision were both <11%. The accuracy of the method was between 97.3 and 105.6%. The method was successfully applied to pharmacokinetic study of ardisiacrispin A after intravenous administration in rats.

Keywords

UPLC-MS/MS; ardisiacrispin A; pharmacokinetics; rat plasma.

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