1. Academic Validation
  2. 8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice

8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice

  • Am J Physiol Regul Integr Comp Physiol. 2016 Dec 1;311(6):R1022-R1031. doi: 10.1152/ajpregu.00521.2015.
Rie Mukai 1 Hitomi Horikawa 2 Pei-Yi Lin 2 Nao Tsukumo 2 Takeshi Nikawa 3 Tomoyuki Kawamura 4 Hisao Nemoto 4 Junji Terao 2
Affiliations

Affiliations

  • 1 Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; rmukai@tokushima-u.ac.jp.
  • 2 Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • 3 Department of Nutritional Physiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; and.
  • 4 Department of Pharmaceutical Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Abstract

8-Prenylnaringenin (8-PN) is a prenylflavonoid that originates from hop extracts and is thought to help prevent disuse muscle atrophy. We hypothesized that 8-PN affects muscle plasticity by promoting muscle recovery under disuse muscle atrophy. To test the promoting effect of 8-PN on muscle recovery, we administered an 8-PN mixed diet to mice that had been immobilized with a cast to one leg for 14 days. Intake of the 8-PN mixed diet accelerated recovery from muscle atrophy, and prevented reductions in Akt phosphorylation. Studies on cell cultures of mouse myotubes in vitro demonstrated that 8-PN activated the PI3K/Akt/P70S6K1 pathway at physiological concentrations. A cell-culture study using an inhibitor of estrogen receptors and an in vivo experiment with ovariectomized mice suggested that the estrogenic activity of 8-PN contributed to recovery from disuse muscle atrophy through activation of an Akt phosphorylation pathway. These data strongly suggest that 8-PN is a naturally occurring compound that could be used as a nutritional supplement to aid recovery from disuse muscle atrophy.

Keywords

8-prenylnaringenin; IGF1/PI3K/Akt pathway; disuse muscle atrophy; nutritional supplement; phosphorylation.

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