2. Academic Validation
  3. Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis

Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis

  • Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):278-284. doi: 10.1016/j.bbrc.2017.10.040.
Zhao Deng 1,  Pei Luo 2,  Wen Lai 1,  Tongxing Song 1,  Jian Peng 1,  Hong-Kui Wei 3
Affiliations

Affiliations

  • 1 Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
  • 2 Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China.
  • 3 Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China. Electronic address: weihongkui@mail.hzau.edu.cn.
PMID: 29024627 DOI: 10.1016/j.bbrc.2017.10.040
Abstract

Growth of skeletal muscle is dependent on the protein synthesis, and the rate of protein synthesis is mainly regulated in the stage of translation initiation and elongation. Myostatin, a member of the Transforming Growth Factor-β (TGF-β) superfamily, is a negative regulator of protein synthesis. C2C12 myotubes was incubated with 0, 0.01, 0.1, 1, 2, 3 μg/mL myostatin recombinant protein, and then we detected the rates of protein synthesis by the method of SUnSET. We found that high concentrations of myostatin (2 and 3 μg/mL) inhibited protein synthesis by blocking mTOR and eEF2K-eEF2 pathway, while low concentration of myostatin (0.01, 0.1 and 1 μg/mL) regulated eEF2K-eEF2 pathway activity to block protein synthesis without affected mTOR pathway, and myostatin inhibited eEF2K-eEF2 pathway through regulating AMPK pathway to suppress protein synthesis. It provided a new mechanism for myostatin regulating protein synthesis and treating muscle atrophy.

Keywords

AMPK; Myostatin; Protein synthesis; eEF2K-eEF2.

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