1. Academic Validation
  2. Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

  • Food Chem. 2019 Mar 15;276:768-775. doi: 10.1016/j.foodchem.2018.10.045.
Kyari Yates 1 Franziska Pohl 1 Maike Busch 2 Annika Mozer 2 Louis Watters 3 Andrey Shiryaev 4 Paul Kong Thoo Lin 5
Affiliations

Affiliations

  • 1 School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, United Kingdom.
  • 2 School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, United Kingdom; Department of Natural Science, Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.
  • 3 School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, United Kingdom; Artesis Plantijn University College Antwerp, Belgium.
  • 4 Chemistry Department, Samara State Technical University, Russia.
  • 5 School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, United Kingdom. Electronic address: p.v.s.kong-thoo-lin@rgu.ac.uk.
Abstract

Sinapine is the main secondary metabolite present in rapeseed pomace (RSP) with its concentration being dependent on rapeseed processing, growing conditions, extraction parameters and the country of origin. Here we report, the concentration of sinapine from an extract of defatted RSP harvested in the North East of Scotland. Using liquid chromatography tandem mass spectrometry, the most abundant phenolic compound in the RSP extract was, as expected, sinapine (109.1 mg/g RSP extract). Additionally, sinapic, caffeic, ferulic and syringic acids were identified (0.159-3.91 mg/g RSP extract). Sinapine together with the phenolics at the concentration present in the RSP extract, exhibited ≥50% activity relative to the extract in antioxidant assays. Furthermore, sinapine provided plasmid DNA (pBR322) protection, from 2,2'-azobis(2-amidinopropane) dihydrochloride and inhibited acetylcholinesterase activity by 85%. Molecular docking was utilised to explain the inhibitory activity. RSP can be an excellent source of bioactive compounds for pharmaceuticals, food additive and nutraceutical applications.

Keywords

Acetylcholinesterase (AChE); Antioxidant assays; Canola; LC-MS/MS; Phenolic acids; Plasmid DNA (pBR322); Rapeseed pomace; Sinapine.

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