1. Academic Validation
  2. Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells

Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells

  • Mol Ther Nucleic Acids. 2019 Jun 7;16:105-117. doi: 10.1016/j.omtn.2019.02.008.
Shu-Qing Wang 1 Xiang-Yu Yang 2 Xin-Feng Yu 1 Shu-Xiang Cui 3 Xian-Jun Qu 4
Affiliations

Affiliations

  • 1 Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • 2 Department of Stomatology, Aerospace Center Hospital, Haidian District, Beijing, China.
  • 3 Beijing Key Laboratory of Environmental Toxicology, Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China. Electronic address: sxccui@ccmu.edu.cn.
  • 4 Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. Electronic address: quxj@ccmu.edu.cn.
Abstract

The important role of insulin-like growth factor-1 receptor (IGF-1R) in tumorigenesis has been well established. The classical model involves IGF-1R binding to IGF-1/2, the following activation of PI3K-Akt-signaling cascades, driving cell proliferation and Apoptosis inhibition. Here we report a new signal transduction pathway of IGF-1R in the intestinal epithelium. Using heterozygous knockout mice (Igf1r+/-), we analyzed the expressions of viral RNA sensors MDA5 and RIG-I in the intestinal epithelium. Igf1r+/- mice exhibited higher MDA5 and RIG-I than wild-type (WT) mice, indicating that knockdown of IGF-1R could trigger MDA5 and RIG-I. IGF-1R knockdown-triggered MDA5 and RIG-I were further investigated in human colonic Cancer cells. Increased MDA5 and RIG-I were clearly seen in the cytoplasm in Cancer cells as well as normal human colonic cells with silenced IGF-1R. Notably, the upregulations of MDA5 and RIG-I was not affected by blockage of the PI3K-Akt pathway with LY294002. These results suggested a new signal transduction pathway of IGF-1R. Importantly, IGF-1R knockdown-triggered MDA5 and RIG-I resulted in colorectal cancer Apoptosis through activation of the mitochondrial pathway. These in vitro observations were evidenced in the azoxymethane (AOM)-dextran sulfate sodium (DSS) colorectal Cancer model of mice. In conclusion, knockdown of IGF-1R triggers viral RNA sensor MDA5- and RIG-I-mediated mitochondrial Apoptosis in Cancer cells.

Keywords

IGF-1R; MDA5; RIG-I; mitochondrial apoptosis; viral RNA sensors.

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