1. Academic Validation
  2. Lovastatin attenuates angiotensin II induced cardiovascular fibrosis through the suppression of YAP/TAZ signaling

Lovastatin attenuates angiotensin II induced cardiovascular fibrosis through the suppression of YAP/TAZ signaling

  • Biochem Biophys Res Commun. 2019 May 14;512(4):736-741. doi: 10.1016/j.bbrc.2019.03.158.
Pei Wu 1 Zhenzhen Liu 1 Tingting Zhao 1 Fan Xia 1 Li Gong 1 Zeqi Zheng 2 Zihua Chen 3 Tianlun Yang 4 Qiong Duan 5
Affiliations

Affiliations

  • 1 Department of Cardiology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, China.
  • 2 Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China; Jiangxi Hypertension Research Institute, Nanchang, China.
  • 3 Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.
  • 4 Department of Cardiology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, China. Electronic address: tianluny@163.com.
  • 5 Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China; Jiangxi Hypertension Research Institute, Nanchang, China. Electronic address: qiongduan@csu.edu.cn.
Abstract

Angiotensin II (ANG II) is associated with fibrosis in both clinical and basic studies. Thus, we aimed to explore a mechanism by which ANG II induces fibrosis. 5 μM of ANG II was used in the in vitro study. The mouse cardiovascular fibrosis model was established by infused with AngII (1000 ng/kg/min) for 7 days and cotreated with lovastatin (10 mg/kg daily) or vehicle control (DMSO in saline). We found that ANG II activated yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), two transcription factors that were shown to induce fibrosis. Inhibition of Ras homolog gene family member A (RhoA) reduced ANG II-induced YAP/TAZ transcriptional activity, which suggests that the upregulation of YAP/TAZ signaling by ANG II is RhoA-dependent. Furthermore, studies have shown that the inhibition of YAP/TAZ by either siRNA or small molecule inhibitor suppressed ANG II-induced expression of fibrogenic genes, indicating that ANG II upregulates YAP/TAZ to initiate fibrosis. The mevalonate pathway, which is targeted by statins, has also been shown to control YAP/TAZ. Here, we found that the suppression of YAP/TAZ signaling by lovastatin attenuates ANG II-induced fibrosis, both in vitro and in vivo. These data reveal a novel mechanism for ANG II in the induction of fibrosis. In addition, our findings provide a reasonable explanation regarding the mechanism by which statins improve fibrosis in patients with cardiovascular and renal diseases.

Keywords

Angiotensin II; Fibrosis; Hippo signaling; Mevalonate pathway; RhoA.

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