1. Academic Validation
  2. Long Noncoding RNA NEAT1 Aggravates Aβ-Induced Neuronal Damage by Targeting miR-107 in Alzheimer's Disease

Long Noncoding RNA NEAT1 Aggravates Aβ-Induced Neuronal Damage by Targeting miR-107 in Alzheimer's Disease

  • Yonsei Med J. 2019 Jul;60(7):640-650. doi: 10.3349/ymj.2019.60.7.640.
Sha Ke 1 Zhaohui Yang 2 Fei Yang 1 Xiaoming Wang 1 Juan Tan 1 Bo Liao 3
Affiliations

Affiliations

  • 1 Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.
  • 2 Department of Burn and Plastic Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.
  • 3 Department of Urology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China. liaobonsmc@163.com.
Abstract

Purpose: Alzheimer's disease (AD) is the most common neurodegenerative disease, with a rising prevalence worldwide. Long noncoding RNAs (lncRNAs) have been found to play important roles in the development and treatment of AD. However, the exact role of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in neuronal damage in AD is largely unknown.

Materials and methods: The AD model was established in SH-SY5Y and SK-N-SH cells via treatment with amyloid β1-42 (Aβ). The expression of NEAT1 and microRNA-107 (miR-107) was measured by quantitative real-time polymerase chain reaction. Cell viability and Apoptosis were detected by MTT assay, immunocytochemistry, and flow cytometry. The expression of phosphorylated Tau Protein (p-Tau) was measured by Western blot. The interaction between NEAT1 and miR-107 was explored by bioinformatics analysis, luciferase activity, and RNA immunoprecipitation assays.

Results: NEAT1 expression was enhanced in Aβ-treated SH-SY5Y and SK-N-SH cells, and its knockdown attenuated Aβ-induced inhibition of viability and promotion of Apoptosis and p-Tau levels. NEAT1 was indicated as a decoy of miR-107. miR-107 abundance was reduced in Aβ-treated cells, and its overexpression reversed Aβ-induced injury. Moreover, interference of miR-107 abated silencing of NEAT1-mediated inhibition of neuronal damage in Aβ-treated SH-SY5Y and SK-N-SH cells.

Conclusion: LncRNA NEAT1 aggravated Aβ-induced neuronal damage by sponging miR-107, indicating a novel avenue for treatment of AD.

Keywords

Alzheimer's disease; NEAT1; miR-107; neuronal damage.

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