1. Academic Validation
  2. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes

Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes

  • Inflammation. 2020 Jun;43(3):916-936. doi: 10.1007/s10753-020-01178-0.
Li Qiao 1 Yi Li 1 Shui Sun 2
Affiliations

Affiliations

  • 1 Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
  • 2 Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China. sunshuisph1965@163.com.
Abstract

Osteoarthritis (OA) is considered the most frequent degenerative disease and is characterized by cartilage degradation and synovial inflammation. Fibroblast-like synoviocytes (FLSs) are vital to synovial inflammation in OA. Type 2 diabetes mellitus (T2DM) is characterized by Insulin resistance and hyperinsulinemia (HINS) and has been demonstrated to be an independent risk factor for OA. Autophagy is involved in the processes of various inflammatory diseases, and Autophagy inhibition can stimulate OA development. Thus, we aimed to investigate the role of Insulin in the inflammatory phenotype and Autophagy of FLSs in OA. The data showed that cell viability and proinflammatory cytokine production in FLSs were both increased after Insulin stimulation. We also found that high Insulin could promote macrophage infiltration and chemokine production but inhibited Autophagy in FLSs. To further explore the potential mechanisms, the effects of Insulin on PI3K/Akt/mTOR and NF-ĸB signaling activation were evaluated. The results indicated that Insulin activated PI3K/Akt/mTOR/NF-ĸB signaling, and the above-mentioned inflammatory responses, including Autophagy inhibition, were notably attenuated by specific signaling inhibitors in the presence of high Insulin. Moreover, the data showed that a positive feedback loop existed between proinflammatory cytokines (e.g., IL-1β, IL-6, and TNF-α) and PI3K/mTOR/Akt/NF-ĸB signaling in FLSs, and Insulin enhanced this feedback loop to accelerate OA progression. Our study suggests that Insulin may be a novel therapeutic strategy for OA prevention and treatment in the future.

Keywords

autophagy; fibroblast-like synoviocytes; inflammation; insulin; osteoarthritis.

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