1. Academic Validation
  2. Multivalent and synergistic chitosan oligosaccharide-Ag nanocomposites for therapy of bacterial infection

Multivalent and synergistic chitosan oligosaccharide-Ag nanocomposites for therapy of bacterial infection

  • Sci Rep. 2020 Jun 19;10(1):10011. doi: 10.1038/s41598-020-67139-7.
Lin Mei 1 Zhenlong Xu 2 Yanmei Shi 3 Chunlei Lin 2 Shuyan Jiao 2 Lijun Zhang 4 Pengxu Li 4
Affiliations

Affiliations

  • 1 School of Materials and Chemical Engineering, Zhongyuan University of Technology, Zhengzhou, 450007, P.R, China. lins79@sina.com.
  • 2 School of Materials and Chemical Engineering, Zhongyuan University of Technology, Zhengzhou, 450007, P.R, China.
  • 3 Scientific Research Center, Henan University of Chinese Medicine, Zhengzhou, 450046, P.R, China.
  • 4 College of International Education, Zhongyuan University of Technology, Zhengzhou, 450007, P.R, China.
Abstract

Chitosan oligosaccharide functionalized silver nanoparticles with synergistic Bacterial activity were constructed as a multivalent inhibitor of bacteria. Placing the chitosan oligosaccharide on silver nanoparticles can dramatically enhance the adsorption to the Bacterial membrane via multivalent binding. The multicomponent nanostructures can cooperate synergistically against gram-positive and gram-negative bacteria. The Antibacterial activity was increased via orthogonal array design to optimize the synthesis condition. The synergistic Bacterial activity was confirmed by fractional inhibitory concentration and zone of inhibition test. Through studies of antimicrobial action mechanism, it was found that the nanocomposites interacted with the bacteria by binding to Mg2+ ions of the Bacterial surface. Then, the nanocomposites disrupted Bacterial membrane by increasing the permeability of the outer membrane, resulting in leakage of cytoplasm. This strategy of chitosan oligosaccharide modification can increase the Antibacterial activity of silver nanoparticles and accelerate wound healing at the same time. The nanomaterial without cytotoxicity has promising applications in bacteria-infected wound healing therapy.

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