2. Academic Validation
  3. PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation

  • Mol Cell. 2021 Apr 1;81(7):1425-1438.e10. doi: 10.1016/j.molcel.2021.01.032.
Yuqian Shen 1,  Zi Chao Zhang 2,  Shanshan Cheng 1,  An Liu 1,  Jian Zuo 1,  Shuting Xia 3,  Xian Liu 1,  Wenhua Liu 1,  Zhengping Jia 4,  Wei Xie 5,  Junhai Han 6
Affiliations

Affiliations

  • 1 School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China.
  • 2 School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China. Electronic address: zhangzc@seu.edu.cn.
  • 3 Institute of Neuroscience, Soochow University, Suzhou 215000, China.
  • 4 Neurosciences and Mental Health Program, Hospital for Sick Children, University of Toronto, Toronto, ON M5S 1A1, Canada.
  • 5 School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
  • 6 School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China; Department of Neurology, Affiliated ZhongDa Hospital, Institute of Neuropsychiatry, Southeast University, Nanjing, Jiangsu 210009, China. Electronic address: junhaihan@seu.edu.cn.
PMID: 33662272 DOI: 10.1016/j.molcel.2021.01.032
Abstract

Eukaryotic elongation factor 2 (eEF2) mediates translocation of peptidyl-tRNA from the ribosomal A site to the P site to promote translational elongation. Its phosphorylation on Thr56 by its single known kinase eEF2K inactivates it and inhibits translational elongation. Extensive studies have revealed that different signal cascades modulate eEF2K activity, but whether additional factors regulate phosphorylation of eEF2 remains unclear. Here, we find that the X chromosome-linked intellectual disability protein polyglutamine-binding protein 1 (PQBP1) specifically binds to non-phosphorylated eEF2 and suppresses eEF2K-mediated phosphorylation at Thr56. Loss of PQBP1 significantly reduces general protein synthesis by suppressing translational elongation. Moreover, we show that PQBP1 regulates hippocampal metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) and mGluR-LTD-associated behaviors by suppressing eEF2K-mediated phosphorylation. Our results identify PQBP1 as a novel regulator in translational elongation and mGluR-LTD, and this newly revealed regulator in the eEF2K/eEF2 pathway is also an excellent therapeutic target for various disease conditions, such as neural diseases, virus Infection, and Cancer.

Keywords

PQBP1; Renpenning syndrome; eEF2; mGluR-LTD; novel object recognition; phosphorylation; protein synthesis; spatial recognition; translational elongation.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z