1. Academic Validation
  2. Nr4a1 promotes cell adhesion and fusion by regulating Zeb1 transcript levels in myoblasts

Nr4a1 promotes cell adhesion and fusion by regulating Zeb1 transcript levels in myoblasts

  • Biochem Biophys Res Commun. 2021 Jun 4;556:127-133. doi: 10.1016/j.bbrc.2021.03.153.
Yixuan Liu 1 Nanqi Liu 2 Yang Yu 3 Difei Wang 4
Affiliations

Affiliations

  • 1 Department of Gerontology, ShengJing Hospital of China Medical University, Shenyang, Liaoning, 110004, China.
  • 2 Institute of Health Science, China Medical University, Shenyang, Liaoning, 110122, China.
  • 3 Key Laboratory of Medical Cell Biology, Institute of Translational Medicine, China Medical University, Shenyang, Liaoning, 110122, China.
  • 4 Department of Gerontology, ShengJing Hospital of China Medical University, Shenyang, Liaoning, 110004, China. Electronic address: dfwang@cmu.edu.cn.
Abstract

Nuclear receptor subfamily 4 group A member 1 (NR4A1) acts as a myogenic factor in muscle development and regeneration; however, it remains unclear how Nr4a1 regulates myoblast physiology. In this study, report a role for Nr4a1-mediated regulation of cell adhesion in myoblast and muscle tissue. Nr4a1-overexpression myoblast, Nr4a1-konckdown myoblast and mice gastrocnemius muscle following an injection with an adenovirus vector expression Nr4a1 (Nr4a1-AAV) were used to observe the changes in cell adhesion. Nr4a1 was found to enhance cell-cell contact and adhesion molecule expression in myoblasts. In contrast, the deletion of Nr4a1 expression inhibited junction and adhesion between myoblasts. Moreover, Nr4a1 increased myoblast adhesion via directly binding to an upstream site of zinc finger E-box binding homeobox 1 (Zeb1), which is required for myogenesis in myoblasts. In mice, Zeb1 induced increased cadherin and Integrin expression in the gastrocnemius muscle following an injection with an adenovirus vector expressing Nr4a1(Nr4a1-AAV). These data indicate that Nr4a1 regulates myoblast adhesion via Zeb1 expression.

Keywords

Cell adhesion; Myoblast; Nr4a1; Zeb1.

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