2. Academic Validation
  3. Duck Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent Cellular Translation Shutoff via PERK/GCN2

Duck Hepatitis A Virus Type 1 Induces eIF2α Phosphorylation-Dependent Cellular Translation Shutoff via PERK/GCN2

  • Front Microbiol. 2021 Apr 12;12:624540. doi: 10.3389/fmicb.2021.624540.
Yuanzhi Liu 1 2 3,  Anchun Cheng 1 2 3,  Mingshu Wang 1 2 3,  Sai Mao 1 2 3,  Xumin Ou 1 2 3,  Qiao Yang 1 2 3,  Ying Wu 1 2 3,  Qun Gao 1 2 3,  Mafeng Liu 1 2 3,  Shaqiu Zhang 1 2 3,  Juan Huang 1 2 3,  Renyong Jia 1 2 3,  Dekang Zhu 2 3,  Shun Chen 1 2 3,  Xinxin Zhao 1 2 3,  Yanling Yu 1 2 3,  Yunya Liu 1 2 3,  Ling Zhang 1 2 3,  Bin Tian 1 3,  Leichang Pan 1 3
Affiliations

Affiliations

  • 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
  • 2 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
  • 3 Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
PMID: 33912143 DOI: 10.3389/fmicb.2021.624540
Abstract

Duck hepatitis A virus type 1 (DHAV-1) is one of the most deadly pathogens that endanger the duck industry. Most viruses usually turn off host translation after Infection to facilitate viral replication and translation. For the first time report to our knowledge, DHAV-1 can induce eIF2α phosphorylation and inhibit cellular translation in duck embryo fibroblasts (DEFs). Moreover, the activity of DHAV-1 in the cells caused obvious eIF2α phosphorylation, which has nothing to do with the viral protein. Subsequently, we screened two kinases (PERK and GCN2) that affect eIF2α phosphorylation through inhibitors and shRNA. Notably, the role of GCN2 in other picornaviruses has not been reported. In addition, when the phosphorylation of eIF2α induced by DHAV-1 is inhibited, the translation efficiency of DEFs restores to a normal level, indicating that DHAV-1 induced cellular translation shutoff is dependent on eIF2α phosphorylation.

Keywords

GCN2; PERK; duck hepatitis A virus type 1; eIF2α; translation shutoff.

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