1. Academic Validation
  2. Circ_0001666 affects miR-620/WNK2 axis to inhibit breast cancer progression

Circ_0001666 affects miR-620/WNK2 axis to inhibit breast cancer progression

  • Genes Genomics. 2021 Aug;43(8):947-959. doi: 10.1007/s13258-021-01114-y.
Na Su 1 Li Liu 2 Shan He 1 Linghai Zeng 3
Affiliations

Affiliations

  • 1 Department of Clinical Laboratory, The First People's Hospital of Jingmen, No. 67 Xiangshan Avenue, Jingmen, 448000, Hubei, China.
  • 2 Department of Nephropathy, Jingmen No. 2 People's Hospital, Jingmen, Hubei, China.
  • 3 Department of Clinical Laboratory, The First People's Hospital of Jingmen, No. 67 Xiangshan Avenue, Jingmen, 448000, Hubei, China. glbxrw@163.com.
Abstract

Background: Circular RNAs (circRNAs) play important roles in the progression of various cancers, including breast Cancer (BC). However, the role of circ_0001666 in BC remains unclear.

Objective: To explore the role of circ_0001666 in the progression of BC and reveal its potential molecular mechanism.

Methods: Real-time polymerase chain reaction was conducted to determine the expression of circ_0001666, miR-620 and with-no-lysine kinase 2 (WNK2). Cell counting kit 8 assay, flow cytometry and transwell assay were used to measure cell proliferation, Apoptosis, migration and invasion. Western blot was utilized to examine the level of protein. Dual-luciferase reporter assay and RNA immunoprecipitation assay were used to verify the interaction between miR-620 and circ_0001666 or WNK2. Mice xenotransplantation models were built to explore the effect of circ_0001666 on BC tumor growth in vivo.

Results: Circ_0001666 was downregulated in BC tumor tissues and cells. Overexpressed circ_0001666 inhibited the proliferation, migration, invasion, while promoted Apoptosis and tumor growth of BC in vitro or in vivo. Furthermore, circ_0001666 could serve as a Sponge of miR-620. MiR-620 inhibitor hindered BC cell progression, which was similar to the effect of circ_0001666 overexpression. WNK2 was a target of miR-620, and circ_0001666 could Sponge miR-620 to positive regulate WNK2. The knockdown of WNK2 reversed the effect of circ_0001666 overexpression on BC progression.

Conclusion: Circ_0001666 hindered the progression of BC via miR-620/WNK2 axis.

Keywords

Breast cancer; Circ_0001666; MiR-620; WNK2.

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