1. Academic Validation
  2. Long non-coding RNA PSMA3-AS1 promotes glioma progression through modulating the miR-411-3p/HOXA10 pathway

Long non-coding RNA PSMA3-AS1 promotes glioma progression through modulating the miR-411-3p/HOXA10 pathway

  • BMC Cancer. 2021 Jul 22;21(1):844. doi: 10.1186/s12885-021-08465-5.
Tianzao Huang  # 1 Yingxian Chen  # 2 Yile Zeng 1 Chaoyang Xu 1 Jinzhong Huang 1 Weipeng Hu 1 Xiangrong Chen 1 Huangde Fu 3
Affiliations

Affiliations

  • 1 Department of Neurosurgery, the Second Affiliated Hospital, Fujian Medical University, Quanzhou, 362000, Fujian, China.
  • 2 Department of Neurosurgery, The Jinjiang Municipal Hospital, Quanzhou, Fujian, China.
  • 3 Department of Neurosurgery, The Second Nanning People's Hospital, 13 Dancun Road, Jiangnan District, Nanning, 530031, Guangxi, China. fuhuangde@163.com.
  • # Contributed equally.
Abstract

Background: Glioma is a common type of brain tumor and is classified as low and high grades according to morphology and molecules. Growing evidence has proved that long non-coding RNAs (lncRNAs) play pivotal roles in numerous tumors or diseases including glioma. Proteasome 20S subunit alpha 3 antisense RNA 1 (PSMA3-AS1), as a member of lncRNAs, has been disclosed to play a tumor-promoting role in Cancer progression. However, the role of PSMA3-AS1 in glioma remains unknown. Therefore, we concentrated on researching the regulatory mechanism of PSMA3-AS1 in glioma.

Methods: PSMA3-AS1 expression was detected using RT-qPCR. Functional assays were performed to measure the effects of PSMA3-AS1 on glioma progression. After that, ENCORI ( http://starbase.sysu.edu.cn/ ) database was used to predict potential genes that could bind to PSMA3-AS1, and miR-411-3p was chosen for further studies. The interaction among PSMA3-AS1, miR-411-3p and homeobox A10 (HOXA10) were confirmed through mechanism assays.

Results: PSMA3-AS1 was verified to be up-regulated in glioma cells and promote glioma progression. Furthermore, PSMA3-AS1 could act as a competitive endogenous RNA (ceRNA) for miR-411-3p to regulate HOXA10 and thus affecting glioma progression.

Conclusion: PSMA3-AS1 stimulated glioma progression via the miR-411-3p/HOXA10 pathway, which might offer a novel insight for the therapy and treatment of glioma.

Keywords

Glioma; HOXA10; PSMA3-AS1; miR-411-3p.

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