1. Academic Validation
  2. MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica

MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica

  • Insect Biochem Mol Biol. 2022 Mar;142:103727. doi: 10.1016/j.ibmb.2022.103727.
Tian-Feng Duan 1 Shu-Jing Gao 2 Hai-Chao Wang 1 Ling Li 1 Yan-Yan Li 1 Yao Tan 1 Bao-Ping Pang 3
Affiliations

Affiliations

  • 1 Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
  • 2 Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
  • 3 Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: pangbp@imau.edu.cn.
Abstract

MicroRNAs (miRNAs) regulate various biological processes in insects. However, their roles in the regulation of insect diapause remain unknown. In this study, we address the biological function of a conserved miRNA, let-7-5p in the regulation of a juvenile hormone primary response gene, Krüppel homolog 1 (Kr-h1), which modulates reproductive diapause in Galeruca daurica. The dual luciferase reporter assay showed that let-7-5p depressed the expression of Kr-h1. The expression profiles of let-7-5p and Kr-h1 displayed opposite patterns in the adult developmental stage. Injection of let-7-5p agomir in pre-diapause adult females inhibited the expression of Kr-h1, which consequently led to delay ovarian development, increase lipid accumulation, expand fat body, and induce reproductive diapause just as depleting Kr-h1 did. Conversely, injection of let-7-5p antagomir resulted in opposite effects by reducing fat storage and stimulating reproduction. Moreover, JH receptor agonist methoprene reduced the expression of let-7-5p, and rescued the ovarian development defects associated with let-7-5p overexpression. These results indicate that let-7-5p plays an important role in the regulation of reproductive diapause and development of G. daurica adults through its target gene Kr-h1.

Keywords

Diapause; Galeruca daurica; Juvenile hormone; Kr-h1; microRNA.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-B1161
    ≥98.0%, Juvenile Hormone Agonist