1. Academic Validation
  2. HDAC6-dependent deacetylation of TAK1 enhances sIL-6R release to promote macrophage M2 polarization in colon cancer

HDAC6-dependent deacetylation of TAK1 enhances sIL-6R release to promote macrophage M2 polarization in colon cancer

  • Cell Death Dis. 2022 Oct 21;13(10):888. doi: 10.1038/s41419-022-05335-1.
Guangying Xu # 1 2 Liling Niu # 1 2 Youhui Wang 1 2 Guang Yang 1 Xingwu Zhu 1 2 Yuan Yao 1 2 Gang Zhao 1 2 Shaowei Wang 1 2 Hui Li 3 4
Affiliations

Affiliations

  • 1 Department of Gastrointestinal Cancer Biology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin, China.
  • 2 Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China.
  • 3 Department of Gastrointestinal Cancer Biology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin, China. lihui05@tmu.edu.cn.
  • 4 Key Laboratory of Cancer Immunology and Biotherapy, Tianjin, China. lihui05@tmu.edu.cn.
  • # Contributed equally.
Abstract

Histone deacetylase 6 (HDAC6), a member of the HDAC family, has been identified as a potential therapeutic target for tumor therapy, but the function and underlying mechanisms of HDAC6 in colon Cancer are incompletely characterized. Our study showed that the infiltration ratio of M2 macrophages was increased in colon Cancer tissues with high HDAC6 expression. Similarly, the knockdown of HDAC6 in colon Cancer cells inhibited cocultured macrophage M2 polarization in vitro. Analysis of the antibody chip revealed that HDAC6 promoted sIL-6R release to enhance macrophage M2 polarization. Mass spectrometry and immunoprecipitation demonstrated that, mechanistically, HDAC6 interacted with transforming growth factor β-activated kinase 1 (TAK1), deacetylated TAK1 at T178 and promoted TAK1 phosphorylation. TAK1-p38 MAPK signaling could further increase the phosphorylation and activity of ADAM17, which is responsible for shedding of IL-6R. Notably, the expression of phosphorylated TAK1 was positively correlated with HDAC6 expression and macrophage M2 polarization in human colon Cancer tissues. Our study revealed a new HDAC6-TAK1-ADAM17 regulatory axis that mediates sIL-6R release and macrophage polarization in colon Cancer.

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