2. Academic Validation
  3. Exosomal circ_0091741 promotes gastric cancer cell autophagy and chemoresistance via the miR-330-3p/TRIM14/Dvl2/Wnt/β-catenin axis

Exosomal circ_0091741 promotes gastric cancer cell autophagy and chemoresistance via the miR-330-3p/TRIM14/Dvl2/Wnt/β-catenin axis

  • Hum Cell. 2022 Nov 3. doi: 10.1007/s13577-022-00790-6.
Yuan Chen # 1 2,  Haofeng Liu # 2,  Junwei Zou # 3,  Guangxin Cao 2,  Yunfei Li 2,  Chungen Xing 4,  Jindong Wu 5
Affiliations

Affiliations

  • 1 Department of General Surgery, The Second Affiliated Hospital of Soochow University, No. 1055, Sanxiang Road, Gusu District, Soochow, 215000, Jiangsu, People's Republic of China.
  • 2 Department of General Surgery, Tumor Hospital Affiliated to Nantong University (Nantong Tumor Hospital), No. 30, Tongyang North Road, Pingchao Town, Tongzhou District, Nantong, 226361, Jiangsu, People's Republic of China.
  • 3 Department of General Surgery, The Second Affiliated Hospital of Wannan Medical College, Wuhu, 241000, People's Republic of China.
  • 4 Department of General Surgery, The Second Affiliated Hospital of Soochow University, No. 1055, Sanxiang Road, Gusu District, Soochow, 215000, Jiangsu, People's Republic of China. xingcg@suda.edu.cn.
  • 5 Department of General Surgery, Tumor Hospital Affiliated to Nantong University (Nantong Tumor Hospital), No. 30, Tongyang North Road, Pingchao Town, Tongzhou District, Nantong, 226361, Jiangsu, People's Republic of China. wujindong5338@163.com.
  • # Contributed equally.
PMID: 36323918 DOI: 10.1007/s13577-022-00790-6
Abstract

The importance of Cancer cell-released exosomes in the treatment of various cancers has been well-characterized. The current study aims to examine the potential biological functions of gastric Cancer (GC) cell-released exosomes delivering a novel circRNA circ_0091741 in GC and the underlying molecular mechanism. Expression of circ_0091741 was examined in the GC cells, (OXA)-resistant HGC-27 (HGC-27/OXA) cells, and isolated exosomes, after which its downstream miRNA was analyzed. The role and mechanism of the circ_0091741 transmitted by GC cells-derived exosomes in GC cell Autophagy and chemoresistance were assessed using various molecular biological methods. A mouse tumor xenograft model was prepared to discern the effect of circ_0091741 on tumorigenesis in vivo. GC cells and their exosomes were characterized by upregulated circ_0091741 expression. circ_0091741 transferred by GC cell-derived exosomes induced the Autophagy and OXA resistance of GC cells. circ_0091741 obstructed the binding of miR-330-3p to TRIM14 and increased the expression of TRIM14. TRIM14 could cause activation of the Wnt/β-catenin signaling pathway by stabilizing Dvl2. By this mechanism, the Autophagy and OXA resistance of GC cells were augmented. In vivo assay unfolded that orthotopic implantation of exosomal circ_0091741 overexpressed GC cells into nude mice enhanced tumorigenesis. In conclusion, our study emphasized the promotive role of exosomal circ_0091741 in Autophagy and chemoresistance of GC cells, thus laying the basis for the development of novel therapeutic targets for GC treatment.

Keywords

Autophagy; Chemoresistance; Dvl2; Exosome; Gastric cancer; TRIM14; Wnt/β-catenin; circ_0091741; microRNA-330-3p.

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