2. Academic Validation
  3. Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants

Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants

  • Cell Rep. 2023 Apr 4;42(4):112351. doi: 10.1016/j.celrep.2023.112351.
Tao Cheng 1,  Yan-Yi Xing 2,  Cong Liu 1,  Yun-Fei Li 1,  Ying Huang 1,  Xiang Liu 1,  Ying-Jie Zhang 1,  Guo-Qin Zhao 3,  Yang Dong 1,  Xin-Xin Fu 1,  Yi-Meng Tian 1,  Li-Ping Shu 3,  Sean G Megason 4,  Peng-Fei Xu 5
Affiliations

Affiliations

  • 1 Women's Hospital, Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 2 Women's Hospital, Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Hangzhou, Zhejiang, China.
  • 3 Department of Immunology, Guizhou Medical University, Guiyang 550004, China.
  • 4 Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA. Electronic address: megason@hms.harvard.edu.
  • 5 Women's Hospital, Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: pengfei_xu@zju.edu.cn.
PMID: 37018074 DOI: 10.1016/j.celrep.2023.112351
Abstract

Much progress has been made toward generating analogs of early embryos, such as gastruloids and embryoids, in vitro. However, methods for how to fully mimic the cell movements of gastrulation and coordinate germ-layer patterning to induce head formation are still lacking. Here, we show that a regional Nodal gradient applied to zebrafish animal pole explant can generate a structure that recapitulates the key cell movements of gastrulation. Using single-cell transcriptome and in situ hybridization analysis, we assess the dynamics of the cell fates and patterning of this structure. The mesendoderm differentiates into the anterior endoderm, prechordal plate, notochord, and tailbud-like cells along an anterior-posterior axis, and an anterior-posterior-patterned head-like structure (HLS) progressively forms during late gastrulation. Among 105 immediate Nodal targets, 14 genes contain axis-induction ability, and 5 of them induce a complete or partial head structure when overexpressed in the ventral side of zebrafish embryos.

Keywords

CP: Developmental biology; anterior-posterior patterning; explant; head formation; nodal; zebrafish.

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