2. Academic Validation
  3. Heterogeneity and mitochondrial vulnerability configurate the divergent immunoreactivity of human induced microglia-like cells

Heterogeneity and mitochondrial vulnerability configurate the divergent immunoreactivity of human induced microglia-like cells

  • Clin Immunol. 2023 Sep 5;109756. doi: 10.1016/j.clim.2023.109756.
Kousuke Yonemoto 1,  Fumihiko Fujii 1,  Ryoji Taira 1,  Masahiro Ohgidani 2,  Katsuhide Eguchi 1,  Sayaka Okuzono 3,  Yuko Ichimiya 1,  Yuri Sonoda 1,  Pin Fee Chong 1,  Hironori Goto 1,  Hikaru Kanemasa 1,  Yoshitomo Motomura 1,  Masataka Ishimura 1,  Yuhki Koga 1,  Keita Tsujimura 4,  Takao Hashiguchi 5,  Hiroyuki Torisu 6,  Ryutaro Kira 7,  Takahiro A Kato 8,  Yasunari Sakai 9,  Shouichi Ohga 1
Affiliations

Affiliations

  • 1 Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • 2 Department of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Hokkaido, Japan.
  • 3 Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Section of Pediatrics, Department of Medicine, Fukuoka Dental College, Fukuoka, Japan.
  • 4 Group of Brain Function and Development, Neuroscience Institute of the Graduate School of Science, Nagoya University, Aichi, Japan; Research Unit for Developmental Disorders, Institute for Advanced Research, Nagoya University, Aichi, Japan.
  • 5 Laboratory of Medical Virology, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.
  • 6 Section of Pediatrics, Department of Medicine, Fukuoka Dental College, Fukuoka, Japan.
  • 7 Department of Pediatric Neurology, Fukuoka Children's Hospital, Fukuoka, Japan.
  • 8 Department of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • 9 Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. Electronic address: ysakai22q13@gmail.com.
PMID: 37678717 DOI: 10.1016/j.clim.2023.109756
Abstract

Microglia play versatile roles in progression of and protection against neuroinflammatory diseases. Little is known, however, about the mechanisms underlying the diverse reactivity of microglia to inflammatory conditions. We investigated how human induced microglia-like (iMG) cells respond to innate immune ligands. Quantitative PCR showed that poly-I:C and lipopolysaccharide (LPS) activated the expression of IL1B and TNF. Immunoreactivity of iMG did not differ between controls (n = 11) and patients with neuroinflammatory diseases (n = 24). Flow cytometry revealed that CD14high cells expressed interleukin (IL) -1β after LPS treatment. Immunoblotting showed that poly-I:C and LPS differentially activated inflammatory pathways but commonly induced mitochondrial instability and the expression of Pyruvate Kinase isoform M2 (PKM2). Furthermore, a potent stimulator of PKM2 (DASA-58) alleviated IL-1β production after LPS treatment. These data indicate that heterogeneous cell populations and mitochondrial stability underlie the divergent immunoreactivity of human iMG in environments.

Keywords

Divergence; Heterogeneity; Immunoreactivity; Microglia; Mitochondria; and CD14.

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