1. Academic Validation
  2. Bisdemethoxycurcumin alleviates LPS-induced acute lung injury via activating AMPKα pathway

Bisdemethoxycurcumin alleviates LPS-induced acute lung injury via activating AMPKα pathway

  • BMC Pharmacol Toxicol. 2023 Nov 20;24(1):63. doi: 10.1186/s40360-023-00698-3.
Huifang Li # 1 Qi Zou # 1 Xueming Wang 2
Affiliations

Affiliations

  • 1 Department of respiration medicine, Huangzhou District People's Hospital, Huanggang, 438000, Hubei, China.
  • 2 Department of intensive care unit, Huangzhou District People's Hospital, Zhonghuan Road 31, Huanggang, 438000, Hubei, China. xuemingw2022@163.com.
  • # Contributed equally.
Abstract

Objective: Inflammation and oxidative stress contribute to the pathogenesis of acute lung injury (ALI), and subsequently result in rapid deterioration in health. Considering the indispensable role of bisdemethoxycurcumin (BDMC) in inflammation and oxidative stress, the present study aims to examine the effect of BDMC on sepsis-related ALI.

Methods: C57BL/6 mice were administered with BDMC (100 mg/kg) or an equal volume of vehicle, and then injected with lipopolysaccharides (LPS) to induce ALI. We assessed the parameters of lung injury, inflammatory response and oxidative stress in lung tissues. Consistently, the macrophages with or without BDMC treatment were exposed to LPS to verify the effect of BDMC in vitro.

Results: BDMC suppressed LPS-induced lung injury, inflammation and oxidative stress in vivo and in vitro. Mechanistically, BDMC increased the phosphorylation of AMPKα in response to LPS stimulation, and AMPK inhibition with Compound C almost completely blunted the protective effect of BDMC in LPS-treated mice and macrophages. Moreover, we demonstrated that BDMC activated AMPKα via the cAMP/Epac pathway.

Conclusion: Our study identifies the protective effect of BDMC against LPS-induced ALI, and the underlying mechanism may be related to the activation of cAMP/Epac/AMPKα signaling pathway.

Keywords

AMPKα; Acute lung injury; Bisdemethoxycurcumin; Inflammation; Oxidative stress.

Figures
Products