蛋白提取是从生物样本中分离蛋白的过程，为生物学研究、药物开发和临床诊断提供基础。该技术涉及细胞破碎、蛋白溶解和分离等步骤。选择适当的提取缓冲液和方法是关键，以确保蛋白的完整性和活性。提取后的蛋白可用于免疫印迹、质谱分析、酶活性测定等应用，为了深入理解生命活动和疾病机制提供了重要的实验基础。

Protein extraction is the process of separating proteins from biological samples, providing the basis for biological research, drug development and clinical diagnosis. The technology involves steps such as cell disruption, protein solubilization and separation. Selecting the appropriate extraction buffer and method is key to ensure protein integrity and activity. The extracted protein can be used for immunoblotting, mass spectrometry analysis, enzyme activity determination and other applications, providing an important experimental basis for in-depth understanding of life activities and disease mechanisms.