2. 重组蛋白
  3. Enzymes & Regulators
  4. Translocases (EC 7)
  5. ABCA1 蛋白, Human (Sf9, His, Strep, FLAG)

ABCA1 蛋白, Human (Sf9, His, Strep, FLAG)

目录号: HY-P701357
产品使用指南

ABCA1 蛋白响应 ATP 水解，催化特定磷脂从细胞质易位至细胞外/腔膜小叶。它通过迫使磷脂通过其通道和环进入其胞外域中的细长疏水通道来实现磷脂易位。ABCA1 蛋白, Human (Sf9, His, Strep, FLAG) 是重组的 ABCA1 蛋白，由 Sf9 insect cells 表达，带有 N-Strep, C-Flag, N-8*His 标签。ABCA1 蛋白, Human (Sf9, His, Strep, FLAG) 全长 2260 个氨基酸。

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3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (Sf9, His, Strep, FLAG) is the recombinant human-derived ABCA1 protein, expressed by Sf9 insect cells , with N-Strep, C-Flag, N-8*His labeled tag. The total length of ABCA1 Protein, Human (Sf9, His, Strep, FLAG) is 2260 a.a., .
研究背景

ABCA1 蛋白在特定磷脂从细胞质转移到膜的细胞外/腔内小叶的过程中充当关键催化剂，该过程由 ATP 水解驱动。这种活性对于磷脂向载脂蛋白的转移是不可或缺的，从而导致新生高密度脂蛋白（HDL）的形成。ABCA1 表现出比磷脂酰丝氨酸更倾向于转运磷脂酰胆碱，并且可能在细胞内胆固醇流出至载脂蛋白中发挥类似的作用，从而有助于新生 HDL 的生成。该机制涉及磷脂从质膜外表面的易位，迫使它们通过其通道和环进入其细胞外域中的细长疏水隧道。这个复杂的过程对于调节脂质稳态以及维持细胞和全身脂质平衡至关重要。
种属

Human
表达系统

Sf9 insect cells
标签

N-Strep;C-Flag;N-8*His
蛋白编号

O95477 (A2-V2261)
基因 ID

19
蛋白结构
N-term
8*His-Strep
ABCA1 (A2-V2261)
Accession # O95477
Flag
C-term
同用名
ABCA1; ABC1; CERP
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ABCA1 Protein, Human (Sf9, His, Strep, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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