ACSS1 蛋白, Human (P.pastoris, His)

ACSS1 Protein, Human (P.pastoris, His) is a mitochondrial matrix enzyme that is expressed in heart, skeletal muscle and brown adipose tissue. ACSS1 Protein, Human is involved in acetate-mediated epigenetic regulation.

酰基辅酶A短链合成酶（ACSS1、ACSS2、ACSS3）是唯一已知的能够将游离乙酸盐转化为乙酰辅酶A的哺乳动物酶。ACSS1 和 ACSS2 主要使用乙酸盐作为底物，而 ACSS3 优先使用丙酸盐。这些酶的活性部分受到特定赖氨酸位点的乙酰化和脱乙酰化的调节。乙酰化会使 ACSS1 和 ACSS2 失活，而去乙酰化则会重新激活它们。ACSS1 是一种线粒体基质酶，在心脏、骨骼肌和棕色脂肪组织中强烈表达。该亚型主要生成乙酰辅酶 A，乙酰辅酶A 将进入柠檬酸循环，通过氧化为 CO2^[1] 获取能量。

Human

P. pastoris

N-His

Q9NUB1 (38A-689K)

84532  [NCBI]

ASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

Approximately 73.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Moffett JR, et, al. Acetate Revisited: A Key Biomolecule at the Nexus of Metabolism, Epigenetics and Oncogenesis-Part 1: Acetyl-CoA, Acetogenesis and Acyl-CoA Short-Chain Synthetases. Front Physiol. 2020 Nov 12;11:580167.  [Content Brief]