1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ADH 蛋白, Drosophila melanogaster (His, Strep)

ADH 蛋白, Drosophila melanogaster (His, Strep)

目录号: HY-P701939
产品使用指南

ADH 蛋白是氧化还原酶家族中的一种含锌二聚体酶,具有乙醛脱氢酶和醇脱氢酶活性,能够氧化伯醇和仲醇。ADH 蛋白 由大肠杆菌表达且无标签,其起始甲硫氨酸被自然去除。ADH 蛋白, Drosophila melanogaster (His, Strep) 是重组的 ADH 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。ADH 蛋白, Drosophila melanogaster (His, Strep) 全长 255 个氨基酸。

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描述

ADH Protein, Drosophila melanogaster (S2-I256) is a dimeric Zn-containing enzyme in the oxidoreductase family with acetaldehyde dehydrogenase and alcohol dehydrogenase activity, therefore, can oxidize primary and secondary alcohols. ADH Protein, Drosophila melanogaster (S2-I256) is E.coli-sourced and tag-free, the initiator methionine is naturally removed. ADH Protein, Drosophila melanogaster (His, Strep) is the recombinant ADH protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of ADH Protein, Drosophila melanogaster (His, Strep) is 255 a.a., .

研究背景

醇脱氢酶蛋白 (ADHs) 是氧化还原酶家族中的一组含锌二聚体酶,具有乙醛脱氢酶 (乙酰化醛基) 和醇脱氢酶 (NAD+ 氧化醇基) 活性,使 ADHs 能够以 NAD+ 或 NADP+ 为电子受体氧化伯醇或仲醇的 [CH-OH] 基团。ADHs 定位于细胞质或部分蛋白复合体中。ADHs 主要表达于肝脏,也存在于循环系统、消化系统、延伸胚带胚胎、脂肪体、生殖系统等其他结构中。ADHs 受维甲酸、生长激素和糖皮质激素的上调,受雄激素和甲状腺激素的下调[1][2][3]

种属

Others

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P00334 (S2-I256)

基因 ID

3771877

蛋白结构
N-term
Strep-6*His
ADH (S2-I256)
Accession # P00334
C-term
同用名
Adh; Alcohol dehydrogenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ADH Protein, Drosophila melanogaster (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADH Protein, Drosophila melanogaster (His, Strep)
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