Animal-Free Activin A 蛋白, Human/Mouse/Rat (His)

The INHBA protein plays a key role in regulating pituitary function by regulating follicle-stimulating hormone secretion together with activin. Its broad effects span a variety of physiological processes, including hormone secretion, germ cell development, erythrocyte differentiation, insulin secretion, nerve cell survival, embryonic development, and bone growth, depending on unique subunit composition. Animal-Free Activin A Protein, Human/Mouse/Rat (His) is the recombinant human, rat, mouse-derived animal-FreeActivin A protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free Activin A Protein, Human/Mouse/Rat (His) is 116 a.a., with molecular weight of ~13.9 kDa.

INHBA 蛋白在垂体功能的复杂调节中发挥着关键作用，与激活素一起促进抑制和激活促卵泡素分泌的相反动态。抑制素和激活素的广泛影响，以 INHBA 为核心，跨越一系列生理过程，包括下丘脑和垂体激素分泌、性腺激素分泌、生殖细胞发育和成熟、红细胞分化、胰岛素分泌、神经细胞存活、胚胎发育轴向发育和骨骼生长，取决于其独特的亚基组成。值得注意的是，抑制素，例如抑制素 A 和抑制素 B，作为对抗激活素功能的对应物出现。在结构上，INHBA 以二聚体形式存在，通过一个或多个二硫键错综复杂地连接，代表 β-A 亚基的同二聚体。激活素（包括激活素 A、激活素 B 和激活素 AB）的多样性进一步强调了它们的特定亚基组成，影响与 FST 和 FSTL3 等调节蛋白的相互作用。这种错综复杂的相互作用强调了 INHBA 在精心协调的调节网络管理不同生理功能方面的核心作用。

Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is ≤ 0.85 ng/mL. The specific activity of recombinant human Activin A is approximately >1.4 x 10³ IU/mg.

Human; Rat; Mouse

E. coli

C-His

P08476 (G311-S426)

3624  [NCBI]

MGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Approximately 13.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 0.1% sarkosyl in 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.