1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-1 beta/CCL4
  6. Animal-Free CCL4 蛋白, Mouse (His)

Animal-Free CCL4 蛋白, Mouse (His)

目录号: HY-P700165AF
COA 产品使用指南

CCL4 蛋白具有炎症和趋化动力学特性,充当单核因子并自缔合形成同型二聚体。Animal-Free CCL4 蛋白, Mouse (His) 是重组的 无动物源 CCL4 蛋白,由 E. coli 表达,带有 N-His 标签。Animal-Free CCL4 蛋白, Mouse (His) 全长 69 个氨基酸,分子量约为 ~8.64 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
2 μg ¥380 In-stock
5 μg ¥720 询价
10 μg ¥1150 In-stock
20 μg ¥1840 询价
50 μg ¥3500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CCL4 protein, with inflammatory and chemokinetic properties, acts as a monokine and self-associates to form homodimers. Animal-Free CCL4 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeCCL4 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free CCL4 Protein, Mouse (His) is 69 a.a., with molecular weight of ~8.64 kDa.

研究背景

CCL4 蛋白以其炎症和趋化动力学特性为特征,作为单核因子发挥作用并形成同二聚体。

生物活性

Measureby its ability to chemoattract BaF3 cells transfected with human CCR5, The ED50 for this effect is <4 ng/mL.

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

P14097 (A24-N92)

基因 ID

20303  [NCBI]

蛋白结构
N-term
His
CCL4 (A24-N92)
Accession # P14097
C-term
同用名
Ccl4; Mip1b; Scya4; C-C motif chemokine 4; Immune activation protein 2; ACT-2; ACT2; Macrophage inflammatory protein 1-beta; MIP-1-beta; Protein H400; SIS-gamma; Small-inducible cytokine A4
氨基酸序列

APMGSDPPTSCCFSYTSRQLHRSFVMDYYETSSLCSKPAVVFLTKRGRQICANPSEPWVTEYMSDLELN

分子量

Approximately 8.64 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 7.4.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 10 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free CCL4 Protein, Mouse (His)
目录号:
HY-P700165AF
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