Animal-Free IGF2 蛋白, Human (His)

The IGF2 protein is an important member of the insulin-like growth factor family and plays an important role in mammalian growth, fetoplacental development, and adult glucose metabolism. Regulated by placental prolactin, affecting tissue differentiation. Animal-Free IGF2 Protein, Human (His) is the recombinant human-derived animal-FreeIGF2 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free IGF2 Protein, Human (His) is 67 a.a., with molecular weight of ~8.28 kDa.

IGF2 蛋白是胰岛素样生长因子家族的一员，在促进生长和影响胎儿胎盘发育方面发挥着关键作用，特别是作为哺乳动物的主要胎儿生长激素。它受胎盘催乳素调节并有助于组织分化。在成人中，IGF2 参与脂肪组织、骨骼肌和肝脏的葡萄糖代谢。作为整合素的配体，它促进 IGF2 信号传导并正向调节生肌转录因子 MYOD1 的功能，从而控制肌肉终末分化。此外，IGF2 通过增加线粒体呼吸速率来抑制成肌细胞分化并调节新陈代谢。此外，在葡萄糖介导的胰岛素共同分泌中，IGF2的对应物preptin充当葡萄糖介导的胰岛素分泌的生理放大器。值得注意的是，IGF2 具有成骨特性，通过磷酸化 MAPK1 和 MAPK3 增强成骨细胞有丝分裂活性。

Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IGF-II is >3x10⁵ IU/mg.

Human

E. coli

N-His

P01344 (A25-E91)

3481  [NCBI]

AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPAKSE

Approximately 8.28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.