Animal-Free IL-12 beta 蛋白, Human (His)

IL-12 beta protein is a multifunctional cytokine that serves as a growth factor for activated T cells and NK cells, amplifies the lytic activity of NK/lymphokine-activated killer cells, and induces IFN production by resting peripheral blood mononuclear cells -γ. peripheral blood mononuclear cells). Animal-Free IL-12 beta Protein, Human (His) is the recombinant human-derived animal-FreeIL-12 beta protein, expressed by E. coli , with His labeled tag. The total length of Animal-Free IL-12 beta Protein, Human (His) is 306 a.a., with molecular weight of ~35.64 kDa.

IL-12 β 蛋白是一种多功能细胞因子，可作为激活的 T 细胞和 NK 细胞的生长因子，放大 NK/淋巴因子激活的杀伤细胞的裂解活性，并通过静息外周血诱导 IFN-γ 的产生单核细胞（PBMC）。当与 IL23A 结合时，它形成异二聚体细胞因子 IL-23，在先天免疫和适应性免疫中发挥着关键作用。该细胞因子通过与由 IL12RB1 和 IL23R 组成的受体复合物结合来发挥作用，启动 Jak-Stat 信号级联。值得注意的是，IL-23 优先激活记忆 T 细胞而不是幼稚 T 细胞，并促进促炎细胞因子的产生。IL-23 与自身免疫性炎症的关联表明其可能参与自身免疫性炎症疾病，并强调其在肿瘤发生中的重要性。

Human

E. coli

His

P29460 (I23-S328)

3593  [NCBI]

MIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS

Approximately 35.64 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.