Animal-Free IL-18 蛋白, Human (His)

IL-18 Protein, a pro-inflammatory cytokine, plays crucial roles in epithelial barrier repair and polarized immune responses involving Th1 cells and NK cells. Upon binding to IL18R1 and IL18RAP, it forms a signaling ternary complex activating NF-kappa-B, leading to inflammatory mediator synthesis. It synergizes with IL-12 to induce IFNG synthesis in Th1 cells and NK cells. IL-18 is involved in inflammation transduction downstream of pyroptosis, released through the GSDMD pore. At the plasma membrane, IL-18 forms a ternary complex, initiating intracellular signaling, and interacts with TMED10 for translocation and secretion. Animal-Free IL-18 Protein, Human (His) is the recombinant human-derived animal-FreeIL-18 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-18 Protein, Human (His) is 157 a.a., with molecular weight of ~19.2 kDa.

IL-18 蛋白是一种促炎细胞因子，在上皮屏障修复和协调涉及 T 辅助细胞 1 (Th1) 细胞和自然杀伤 (NK) 细胞的极化免疫反应中发挥着关键作用。IL-18 与 IL18R1 和 IL18RAP 结合后，形成信号三元复合物，激活 NF-κ-B，导致炎症介质的合成。它与 IL-12 协同作用，诱导 Th1 细胞和 NK 细胞合成 IFNG。值得注意的是，IL-18 参与细胞焦亡下游的炎症转导，其成熟形式通过gasdermin-D (GSDMD) 孔释放到细胞外环境中。在质膜上，IL-18 与配体结合受体亚基 IL18R1 和信号受体亚基 IL18RAP 形成三元复合物，启动细胞内信号传导。此外，IL-18 与货物受体 TMED10 相互作用，促进其从细胞质易位至内质网-高尔基中间室 (ERGIC) 并随后分泌。

Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED₅₀ for this effect is <5 ng/mL.

Human

E. coli

C-His

Q14116 (Y37-D193)

3606  [NCBI]

MYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Approximately 19.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.