Animal-Free IL-20 蛋白, Human (His)

IL-20 Protein, a pro-inflammatory cytokine secreted by monocytes and keratinocytes, crucially regulates immune responses, inflammation, hemopoiesis, and epidermal cell differentiation. It plays a pivotal role in tissue remodeling, wound healing, and maintaining epithelial homeostasis during infections. Additionally, it impacts actin-mediated functions in neutrophils, inhibiting phagocytosis and migration. Signaling through type I or II IL-20 receptor complexes, it activates JAK2, STAT5, AKT, and ERK1/2, forming a heterotrimeric complex with IL20RA/IL20RB. Animal-Free IL-20 Protein, Human (His) is the recombinant human-derived animal-FreeIL-20 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-20 Protein, Human (His) is 152 a.a., with molecular weight of ~18.47 kDa.

IL-20 蛋白是一种主要由单核细胞和皮肤角质形成细胞分泌的促炎和血管生成细胞因子，在免疫反应、炎症调节、造血以及表皮细胞和角质形成细胞的分化中发挥关键作用。它在组织重塑和伤口愈合过程中发挥着关键作用，有助于在感染和炎症反应期间恢复上皮层稳态，从而维持组织完整性。值得注意的是，IL-20 影响活化中性粒细胞中各种肌动蛋白介导的功能，导致吞噬作用、颗粒胞吐作用和迁移受到抑制。其作用通过 I 型 IL-20 受体复合物（包含 IL20RA 和 IL20RB）介导，或者通过 II 型 IL-20 受体复合物（由 IL22RA1 和 IL20RB 组成）介导。作为动脉生成和血管重塑剂，IL-20 可激活多种信号传导过程，包括 JAK2 和 STAT5 的磷酸化，以及丝氨酸和苏氨酸激酶 AKT 和 ERK1/2 的激活。此外，它与其主要高亲和力异二聚体受体 IL20RA/IL20RB 形成 1:1:1 异三聚体复合物。

Measure by its ability to chemoattract BaF3 cells transfected with human IL-20R alpha and IL-20R beta. The ED₅₀ for this effect is <0.2 ng/mL.

Human

E. coli

C-His

Q9NYY1 (L25-E176)

50604  [NCBI]

MLKTLNLGSCVIATNLQEIRNGFSEIRGSVQAKDGNIDIRILRRTESLQDTKPANRCCLLRHLLRLYLDRVFKNYQTPDHYTLRKISSLANSFLTIKKDLRLCHAHMTCHCGEEAMKKYSQILSHFEKLEPQAAVVKALGELDILLQWMEETE

Approximately 18.47 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.