Animal-Free IL-27 beta/EBI3 蛋白, Human (His)

The IL-27 beta/EBI3 Protein, in conjunction with IL27, forms the IL-27 interleukin, pivotal in innate immunity. Possessing dual pro- and anti-inflammatory properties, IL-27 regulates T-helper cell development, inhibits T-cell proliferation, stimulates cytotoxic T-cell activity, induces B-cell isotype switching, and impacts innate immune cells. Under IL-27 influence, CD4 T-helper cells differentiate into TH1, TH2, and TH17 cells. IL-27 promotes rapid clonal expansion of naive CD4 T-cells, synergizes with IL-12 for IFN-gamma production, and contributes to antitumor and antiangiogenic activities through WSX-1/TCCR receptor activation. It forms heterodimers with IL27/IL27A (IL-27) and IL12A (IL-35), with no disulfide linkage, and interacts with SQSTM1. Animal-Free IL-27 beta/EBI3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-27 beta/EBI3 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-27 beta/EBI3 Protein, Human (His) is 209 a.a., with molecular weight of ~24.25 kDa.

IL-27 beta/EBI3 蛋白与 IL27 结合构成 IL-27 白细胞介素，这是一种异二聚体细胞因子，在先天免疫中发挥着至关重要的作用。IL-27 具有促炎和抗炎特性，可调节 T 辅助细胞发育、抑制 T 细胞增殖、刺激细胞毒性 T 细胞活性、诱导 B 细胞同种型转换，并对先天免疫细胞产生多种影响。CD4 T 辅助细胞在 IL-27 的影响下，可以分化为 1 型效应细胞 (TH1)、2 型效应细胞 (TH2) 和产生 IL-17 的辅助 T 细胞 (TH17)。IL-27 促进初始 CD4 T 细胞（但不包括记忆 CD4 T 细胞）的快速克隆扩增，并与 IL-12 强烈协同作用，诱导初始 CD4 T 细胞产生干扰素-γ/IFN-γ。IL-27 与细胞因子受体 WSX-1/TCCR 结合，有助于抗肿瘤和抗血管生成活性，从而激活抗血管生成趋化因子的产生。它与 IL27/IL27A 形成异二聚体，被识别为白细胞介素 IL-27，并与 IL12A 形成异二聚体，形成白细胞介素 IL-35，两者均未通过二硫键连接。此外，IL-27 与 SQSTM1 相互作用。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.

Human

E. coli

C-His

Q14213 (R21-K229)

10148  [NCBI]

MRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

Approximately 24.25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.