Animal-Free IL-4 蛋白, Human (His)

The IL-4 protein is primarily secreted by mast cells, T cells, eosinophils, and basophils and is critical for antibody production, hematopoiesis, and immune responses. It induces MHC class II expression on B cells, enhances IgE and IgG1 secretion, and modulates CD23 and IL31RA expression. Animal-Free IL-4 Protein, Human (His) is the recombinant human-derived animal-FreeIL-4 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-4 Protein, Human (His) is 129 a.a., with molecular weight of ~15.9 kDa.

细胞因子 IL-4 主要由肥大细胞、T 细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌，在调节抗体产生、造血、炎症和效应 T 细胞反应的发展中发挥着至关重要的作用。IL-4 诱导静息 B 细胞上 II 类 MHC 分子的表达，并增强 IgE 和 IgG1 的分泌和细胞表面表达，从而促进免疫反应。此外，IL-4 调节淋巴细胞和单核细胞上 IgE 低亲和力 Fc 受体 (CD23) 的表达，并正调节巨噬细胞中 IL31RA 的表达。此外，IL-4 通过干扰 mTORC1 信号传导和诱导 RUFY4 来刺激树突状细胞中的自噬。除了其免疫功能之外，IL-4 在正常大脑的高级功能（例如记忆和学习）中发挥着关键作用。与其受体 IL-4R 结合后，IL-4 通过两种类型的受体复合物启动信号传导，1 型主要在造血细胞上，2 型在非造血细胞上，激活 JAK3 并在较小程度上磷酸化 JAK1，从而激活信号转导子和转录激活子 6/STAT6。IL-4 与 IL-4R 和 IL13RA1 相互作用，介导其多种生理效应。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-4 is approximately >2.8 x 10⁷ IU/mg

Human

E. coli

C-His

P05112 (H25-S153)

3565  [NCBI]

MHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Approximately 15.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.