Animal-Free M-CSF 蛋白, Human (His)

The M-CSF protein regulates macrophage production, differentiation, and function. It exists as an active disulfide-linked homodimer outside of cells, generated through proteolytic cleavage. It may also play a role in placental development. Alternative splicing produces multiple transcript variants. M-CSF is widely expressed in tissues such as placenta, spleen, and 25 other tissues. Animal-Free M-CSF Protein, Human (His) is the recombinant human-derived animal-FreeM-CSF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free M-CSF Protein, Human (His) is 232 a.a., with molecular weight of ~13.34 kDa.

M-CSF 蛋白是一种细胞因子，在调节巨噬细胞的产生、分化和功能中发挥关键作用。它以二硫键连接的同二聚体的活性形式存在，存在于细胞外。这种活性形式被认为是通过膜结合前体的蛋白水解裂解产生的。此外，这种蛋白质可能参与胎盘的发育。选择性剪接产生多种转录变体。M-CSF 蛋白在多种组织中广泛表达，包括胎盘 (RPKM 19.9)、脾 (RPKM 19.7) 和 25 种其他组织。

Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human M-CSF is approximately >2.5x10⁸ IU/mg.

Human

E. coli

C-His

NP_757350.2 (E33-S190)

1435  [NCBI]

MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS

18.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.