Animal-Free MCP-1/CCL2 蛋白, Mouse (His)

MCP-1/CCL2 protein serves as a CCR2 ligand, inducing a potent chemotactic response and intracellular calcium mobilization in monocytes and basophils, with no impact on neutrophils or eosinophils. Crucial in neuropathic pain and enhancing NMDA-mediated synaptic transmission in dopamine D1 and D2 receptor-containing neurons, it may involve MAPK/ERK-dependent GRIN2B/NMDAR2B phosphorylation. In monomer or homodimer equilibrium, it binds to endothelial cells via glycosaminoglycan side chains and interacts with TNFAIP6 through its Link domain. Animal-Free MCP-1/CCL2 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeMCP-1/CCL2 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free MCP-1/CCL2 Protein, Mouse (His) is 125 a.a., with molecular weight of ~14.65 kDa.

MCP-1/CCL2 蛋白充当 CC 趋化因子受体 CCR2 的配体，通过 CCR2 结合和激活启动强大的趋化反应和细胞内钙动员。它表现出对单核细胞和嗜碱性粒细胞的趋化活性，同时不影响中性粒细胞或嗜酸性粒细胞。MCP-1/CCL2 在介导周围神经损伤引起的神经性疼痛中发挥着关键作用，还可增强含有多巴胺 D1 和 D2 受体的神经元中 NMDA 介导的突触传递，可能涉及 GRIN2B 的 MAPK/ERK 依赖性磷酸化/NMDAR2B。它以平衡状态下的单体或同型二聚体存在，通过蛋白聚糖的糖胺聚糖 (GAG) 侧链与内皮细胞相连，并通过其 Link 结构域与 TNFAIP6 相互作用。

Measure by its ability to chemoattract BaF3 cells transfected with CCR2A. The ED₅₀ for this effect is <8 ng/mL.

Mouse

E. coli

N-His

P10148 (Q24-N148)

20296  [NCBI]

QPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKLKREVCADPKKEWVQTYIKNLDRNQMRSEPTTLFKTASALRSSAPLNVKLTRKSEANASTTFSTTTSSTSVGVTSVTVN

Approximately 14.65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.