Animal-Free SDF-1 alpha/CXCL12 蛋白, Mouse (His)

The SDF-1 α/CXCL12 protein acts as a chemoattractant with specific activity on T lymphocytes and monocytes (excluding neutrophils). It activates the CXC chemokine receptor CXCR4, inducing a rapid and transient rise in intracellular calcium ions and promoting chemotaxis. Animal-Free SDF-1 alpha/CXCL12 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeSDF-1 alpha/CXCL12 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free SDF-1 alpha/CXCL12 Protein, Mouse (His) is 69 a.a., with molecular weight of ~8.97 kDa.

SDF-1 α/CXCL12 蛋白可作为化学引诱剂，对 T 淋巴细胞和单核细胞（不包括中性粒细胞）具有特异性活性。它激活 CXC 趋化因子受体 CXCR4，诱导细胞内钙离子快速短暂上升并促进趋化性。此外，它还与非典型趋化因子受体 ACKR3 结合，激活 β-arrestin 通路并充当 SDF-1 的清道夫受体。此外，它与整合素的变构位点（位点 2）结合，以不依赖于 CXCR4 的方式激活 ITGAV:ITGB3、ITGA4:ITGB1 和 ITGA5:ITGB1。它通过 LYN 激酶充当单核细胞迁移的正调节剂和单核细胞粘附的负调节剂，通过 CXCR4 和 ACKR3 刺激单核细胞和 T 淋巴细胞的迁移，同时减少单核细胞对 ICAM-1 涂层表面的粘附。SDF1A/CXCR4 信号轴通过 LYN 激酶抑制 β2 整合素 LFA-1 介导的单核细胞与 ICAM-1 的粘附。它在心肌梗塞后发挥保护作用，并诱导ACKR3下调和内化。至关重要的是，SDF-1 alpha/CXCL12 在胚胎发育过程中不可或缺，参与 B 细胞淋巴细胞生成、骨髓中的骨髓生成和心室间隔形成。在 IL7 存在的情况下，它会刺激骨髓来源的 B 细胞祖细胞的增殖，以及基质细胞依赖性前 B 细胞的生长。以单体或同型二聚体形式存在，平衡受到非酸性 pH、多价阴离子的存在以及与 CXCR4 或肝素的结合的影响。单体形式对于完整的趋化活性和抵抗缺血/再灌注损伤至关重要，而二聚体形式作为 CXCR4 的部分激动剂，刺激 Ca2+ 动员而没有趋化活性，而是作为选择性拮抗剂，阻断单体形式诱导的趋化性。它通过变构位点（位点 2）与 ACKR3、整合素亚基 ITGB3 的 N 末端相互作用，并通过 Link 结构域与 TNFAIP6 相互作用。

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED₅₀ for this effect is <0.5 ng/mL.

Mouse

E. coli

C-His

P40224 (G21-K89)

20315  [NCBI]

MGKPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

Approximately 8.97 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.