Animal-Free TGF beta 1/TGFB1 蛋白, Pig (His)

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and regulates its activation through interactions with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS). Animal-Free TGF beta 1/TGFB1 Protein, Pig (His) is the recombinant pig-derived animal-FreeTGF beta 1/TGFB1 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free TGF beta 1/TGFB1 Protein, Pig (His) is 112 a.a., with molecular weight of ~13.7 kDa.

转化生长因子 Beta-1 (TGFB1) 前蛋白是潜伏相关肽 (LAP) 和活性转化生长因子 Beta-1 (TGF-beta-1) 链的前体，构成 TGF-β1 的调节和活性亚基。分别是TGF-β-1。它在细胞外基质储存期间维持 TGF-β-1 链处于潜在状态方面发挥着至关重要的作用。TGFB1 与 TGF-β-1 非共价结合，并通过与“环境分子”（如 LTBP1、LRRC32/GARP 和 LRRC33/NRROS）相互作用来调节其激活，从而控制 TGF-β-1 的激活。值得注意的是，与 LRRC33/NRROS 的相互作用调节巨噬细胞和小胶质细胞中 TGF-β-1 的激活，而与 LRRC32/GARP 的相互作用控制激活的调节性 T 细胞 (Treg) 表面的激活。此外，TGFB1 与整合素（ITGAV:ITGB6 或 ITGAV:ITGB8）的相互作用会诱导潜伏期相关肽链的扭曲，导致随后释放活性 TGF-β-1。

Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells.The ED₅₀ for this effect is<0.1 ng/mL.

Pig

E. coli

C-His

P07200 (A279-S390)

397078  [NCBI]

MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 13.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl pH 4.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 10 mM HCl.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.