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  3. APH1A 蛋白, Human (Cell-Free, His, SUMO)

APH1A 蛋白, Human (Cell-Free, His, SUMO)

目录号: HY-P702211
COA 产品使用指南

APH1A 蛋白是 γ-分泌酶复合物的非催化亚基,对于该内切蛋白酶的组装至关重要。γ-分泌酶复合物,包括早老素同型二聚体、尼卡斯特林、APH1A 和 PSENEN/PEN2,催化膜内蛋白(如 Notch 和 APP)的裂解。APH1A 蛋白, Human (Cell-Free, His, SUMO) 是重组的 APH1A 蛋白,由 E. coli Cell-free 表达,带有 N-6*His, N-SUMO 标签。APH1A 蛋白, Human (Cell-Free, His, SUMO) 全长 247 个氨基酸,分子量为 42.9 kDa。

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规格 价格 是否有货 数量
20 μg ¥5200 In-stock
50 μg ¥9800 In-stock
100 μg   询价  

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描述

The APH1A protein is a noncatalytic subunit of the γ-secretase complex and is essential for the assembly of this endoprotease. The γ-secretase complex, including presenilin homodimer, nicastrin, APH1A, and PSENEN/PEN2, catalyzes the cleavage of intramembrane proteins such as Notch and APP. APH1A Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived APH1A protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of APH1A Protein, Human (Cell-Free, His, SUMO) is 247 a.a., with molecular weight of 42.9 kDa.

研究背景

APH1A 蛋白作为 γ-分泌酶复合物中的非催化亚基,γ-分泌酶复合物是一种内切蛋白酶组装体,对于催化整合膜蛋白(包括 Notch 受体和 APP(淀粉样蛋白-β 前体蛋白))的膜内裂解至关重要。它参与γ-分泌酶组装至关重要,有助于该复合物的正常形成。γ-分泌酶复合物包含早老素同型二聚体(PSEN1 或 PSEN2)、尼卡斯特林 (NCSTN)、APH1(APH1A 或 APH1B)和 PSENEN/PEN2,在各种细胞过程中发挥着关键作用,例如 NotchWnt 信号级联,以及通过处理关键调节蛋白来调节下游事件。此外,它还参与调节胞质 CTNNB1 水平,突出了其在多种细胞途径中的重要性。

种属

Human

表达系统

E. coli Cell-free

标签

N-6*His;N-SUMO

蛋白编号

Q96BI3 (M1-D247)

基因 ID

51107

蛋白结构
N-term
6*His-SUMO
APH1A (M1-D247)
Accession # Q96BI3
C-term
同用名
Gamma-secretase subunit APH-1A; Aph-1alpha; Presenilin-stabilization factor
氨基酸序列

MGAAVFFGCTFVAFGPAFALFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILADALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWYEASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLLCKD

分子量

Observed band size: Monomer: 40 kDa Dimer: 90 kDa It is speculated that the protein forms a dimeric structure.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

APH1A Protein, Human (Cell-Free, His, SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
APH1A Protein, Human (Cell-Free, His, SUMO)
目录号:
HY-P702211
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