ARP3/ACTR3 蛋白, Human (sf9, His-GST)

ARP3/ACTR3 Proteinas, an ATPase preferentially stimulated by single-stranded DNA, crucially participates in homologous recombination repair (HRR). Independent of its ATPase function, it exhibits DNA-binding activity. SWSAP1 forms a functional complex with ZSWIM7, contributing to HRR and mutual protein stabilization. It also interacts with key players in HRR, including RAD51, RAD51B, RAD51C, RAD51D, and XRCC3, emphasizing its role in facilitating DNA repair through homologous recombination mechanisms. ARP3/ACTR3 Protein, Human (sf9, His-GST) is the recombinant human-derived ARP3/ACTR3 protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. The total length of ARP3/ACTR3 Protein, Human (sf9, His-GST) is 417 a.a., with molecular weight of ~75.1 KDa.

SWSAP1 是一种优先受单链 DNA 刺激的 ATP 酶，在同源重组修复 (HRR) 中发挥着至关重要的作用。该蛋白表现出与其 ATP 酶功能无关的 DNA 结合活性。它与 ZSWIM7 形成功能复合物，有助于同源重组修复和蛋白质的相互稳定。SWSAP1 还与同源重组修复过程中的关键参与者相互作用，包括 RAD51、RAD51B、RAD51C、RAD51D 和 XRCC3。这些相互作用凸显了 SWSAP1 通过同源重组机制促进 DNA 修复的作用。

Human

Sf9 insect cells

N-His;N-GST

P61158 (A2-S418)

10096  [NCBI]

Approximately 75.1 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol, 3 mM DTT. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.