1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E2 Enzymes
  5. Autophagy-Related Protein 10 (ATG10)
  6. ATG10 蛋白, Human (GST)

ATG10 蛋白, Human (GST)

目录号: HY-P701246
产品使用指南

ATG10 蛋白, Human (GST)是一种在 E. coli 中表达的,末端有 GST 标签的重组人 Autophagy Related 10 Homolog (ATG10)。ATG10 是一种 E2 泛素结合酶,参与自噬体形成所必需的 2 种泛素样修饰。

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描述

ATG10 Protein, Human (GST) is a recombinant human Autophagy Related 10 Homolog (ATG10) expressed in E. coli with a GST tag. ATG10 is an E2 ubiquitin-conjugating-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation[1].

研究背景

自噬相关蛋白 10 (ATG10) 对于自噬至关重要,因为它促进 ATG5-ATG12 复合物的形成。自噬相关基因10(ATG10)编码E2样酶,与ATG7相互作用,接收泛素样蛋白ATG12,直接识别ATG12和ATG5并催化它们的缀合反应。ATG10 在癌细胞的侵袭和增殖以及细菌感染中发挥着重要作用[1]

种属

Human

表达系统

E. coli

蛋白编号

Q9H0Y0 (M1-P220)

基因 ID

83734

同用名
Ubiquitin-Like-Conjugating Enzyme ATG10; Autophagy-Related Protein 10; APG10-Like; ATG10
氨基酸序列

MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLPMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP

分子量

52.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ATG10 Protein, Human (GST)
目录号:
HY-P701246
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