1. 重组蛋白
  2. Receptor Proteins
  3. AVPR1B 蛋白, Human (Cell-Free, His)

AVPR1B 蛋白, Human (Cell-Free, His)

目录号: HY-P702221
产品使用指南

AVPR1B 蛋白是精氨酸加压素的受体,通过 G 蛋白激活磷脂酰肌醇钙第二信使系统。在 SARS-CoV-2 感染中,AVPR1B 可能识别并内化涉及 AVP/Arg-加压素(SARS-CoV-2 刺突蛋白)的复合物,并通过动力蛋白 2 依赖性内吞作用分泌 ACE2。这表明 AVPR1B 在 SARS-CoV-2 感染的细胞反应中具有潜在作用。AVPR1B 蛋白, Human (Cell-Free, His) 是重组的 AVPR1B 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。AVPR1B 蛋白, Human (Cell-Free, His) 全长 424 个氨基酸,分子量为 53.0 kDa。

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描述

AVPR1B Protein, a receptor for arginine vasopressin, activates a phosphatidyl-inositol-calcium second messenger system through G proteins. In SARS-CoV-2 infection, AVPR1B may recognize and internalize a complex involving AVP/Arg-vasopressin, the SARS-CoV-2 spike protein, and secreted ACE2 via dynamin 2-dependent endocytosis. This suggests a potential role for AVPR1B in the cellular response to SARS-CoV-2 infection. AVPR1B Protein, Human (Cell-Free, His) is the recombinant human-derived AVPR1B protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AVPR1B Protein, Human (Cell-Free, His) is 424 a.a., with molecular weight of 53.0 kDa.

研究背景

AVPR1B 蛋白作为精氨酸加压素的受体,其活性由 G 蛋白介导,导致磷脂酰肌醇钙第二信使系统的激活。此外,在 SARS 冠状病毒 (SARS-CoV-2) 感染期间,AVPR1B 可能会识别并内化由 AVP/Arg-加压素(SARS-CoV-2 刺突蛋白)形成的复合物,并通过动力蛋白 2 依赖性内吞作用分泌 ACE2机制。这种相互作用凸显了 AVPR1B 在 SARS-CoV-2 感染的细胞反应中的潜在作用。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P47901 (M1-F424)

基因 ID

553

蛋白结构
N-term
10*His
AVPR1B (M1-F424)
Accession # P47901
C-term
同用名
Vasopressin V1b receptor; AVPR V1b; AVPR V3; Antidiuretic hormone receptor 1b; Vasopressin V3 receptor
氨基酸序列

MDSGPLWDANPTPRGTLSAPNATTPWLGRDEELAKVEIGVLATVLVLATGGNLAVLLTLGQLGRKRSRMHLFVLHLALTDLAVALFQVLPQLLWDITYRFQGPDLLCRAVKYLQVLSMFASTYMLLAMTLDRYLAVCHPLRSLQQPGQSTYLLIAAPWLLAAIFSLPQVFIFSLREVIQGSGVLDCWADFGFPWGPRAYLTWTTLAIFVLPVTMLTACYSLICHEICKNLKVKTQAWRVGGGGWRTWDRPSPSTLAATTRGLPSRVSSINTISRAKIRTVKMTFVIVLAYIACWAPFFSVQMWSVWDKNAPDEDSTNVAFTISMLLGNLNSCCNPWIYMGFNSHLLPRPLRHLACCGGPQPRMRRRLSDGSLSSRHTTLLTRSSCPATLSLSLSLTLSGRPRPEESPRDLELADGEGTAETIIF

分子量

53.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AVPR1B Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AVPR1B Protein, Human (Cell-Free, His)
目录号:
HY-P702221
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