Carbonic Anhydrase VIII/CA8 蛋白, Mouse (His)

Despite its name, the carbonic anhydrase VIII (CA8) protein lacks carbonic anhydrase catalytic activity. This distinguishes CA8 from its typical functions related to carbonic anhydrase, an enzyme known for its ability to catalyze the reversible hydration of carbon dioxide. Carbonic Anhydrase VIII/CA8 Protein, Mouse (His) is the recombinant mouse-derived Carbonic Anhydrase VIII/CA8 protein, expressed by E. coli , with C-His labeled tag. The total length of Carbonic Anhydrase VIII/CA8 Protein, Mouse (His) is 291 a.a., with molecular weight of ~35 kDa.

根据现有信息，碳酸酐酶 VIII (CA8) 蛋白缺乏碳酸酐酶催化活性，这使其有别于碳酸酐酶家族的其他成员。值得注意的是，CA8 仅在浦肯野细胞中表达，强调了其在中枢神经系统内的细胞特异性分布。CA8 缺乏催化活性表明其作用可能超出与碳酸酐酶相关的经典酶功能。浦肯野细胞中独特的表达模式表明 CA8 在这些神经元中的特殊作用，需要进一步研究其在这种独特的细胞环境中的特定分子和生理功能。阐明 CA8 在浦肯野细胞中的作用可能会为其对神经元过程的贡献以及对神经功能的潜在影响提供有价值的见解。（参考资料：根据现有资料改编，未注明具体出处）

Measured by its ability to hydrolyze 4-nitrophenyl acetate to 4-nitrophenol per minute. The specific activity is 396.0323 pmoL/min/mg.

Mouse

E. coli

C-6*His

P28651 (M1-Q291)

12319  [NCBI]

MADLSFIEDAVAFPEKEEDEEEEEEEGVEWGYEEGVEWGLVFPDANGEYQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLSGGPLPQGQEFELYEVRFHWGRENQRGSEHTVNFKAFPMELHLIHWNSTLFGSIDEAVGKPHGIAIIALFVQIGKEHVGLKAVTEILQDIQYKGKSKTIPCFNPNTLLPDPLLRDYWVYEGSLTIPPCSEGVTWILFRYPLTISQMQIEEFRRLRTHVKGAELVEGCDGILGDNFRPTQPLSDRVIRAAFQ

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.